Compartmentalized embryoid body culture for induction of spatially patterned differentiation

被引：2

作者：

Kaneda, Shohei ^{[1
,2
]}

Kawada, Jiro ^{[1
]}

Akutsu, Hidenori ^{[3
]}

Ichida, Justin ^{[4
,5
]}

Ikeuchi, Yoshiho ^{[6
,7
]}

Fujii, Teruo ^{[1
]}

机构：

[1] Univ Tokyo, Inst Ind Sci, Ctr Int Res Integrat Biomed Syst, Tokyo, Japan

[2] Univ Tokyo, LIMMS CNRS IIS, Inst Ind Sci, Tokyo, Japan

[3] Natl Res Inst Child Hlth & Dev, Dept Reprod Med, Ctr Regenerat Med, Tokyo, Japan

[4] Univ Southern Calif, Keck Sch Med, Dept Stem Cell Biol & Regenerat Med, Los Angeles, CA 90033 USA

[5] Univ Southern Calif, Eli & Edythe Broad CIRM Ctr Regenerat Med & Stem, Los Angeles, CA 90033 USA

[6] Univ Tokyo, Inst Ind Sci, Tokyo, Japan

[7] Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Tokyo, Japan

来源：

BIOMICROFLUIDICS | 2017年 / 11卷 / 04期

关键词：

PLURIPOTENT STEM-CELLS; GRADIENT; MODEL;

D O I：

10.1063/1.4994989

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a compartmentalized culture system of single embryoid bodies (EBs) utilizing a through-hole on a membrane to induce spatially patterned differentiation. An EB derived from mouse pluripotent stem cells was immobilized on the through-hole. By introducing a stem cell maintenance medium and a differentiation medium into upper and lower culture compartments, respectively, a localized differentiated state was achieved only in the lower part of EB, which is exposed to the medium in the lower compartment. This system may enable us to reconstruct complex tissues and to recapitulate developmental processes using EBs. (C) 2017 Author(s).

引用

页数：4

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