A novel combined vaccine against classical swine fever and porcine epidemic diarrhea viruses elicits a significant Th2-favored humoral response in mice

被引：8

作者：

Ding, Yanbin ^{[1
]}

Luo, Lingzhi ^{[1
]}

Luo, Ye ^{[1
]}

Zhao, Dun ^{[1
]}

Mi, Shijiang ^{[2
]}

Yu, Xiaohang ^{[1
]}

Zheng, Jin ^{[1
]}

Tu, Changchun ^{[2
]}

Yu, Xinglong ^{[1
]}

机构：

[1] Hunan Agr Univ, Coll Vet Med, Lab Anim Dis Mol & Immunol, Changsha 410128, Peoples R China

[2] Acad Mil Med Sci, Inst Mil Vet Med, Changchun 130122, Peoples R China

来源：

VACCINE | 2021年 / 39卷 / 33期

关键词：

Combined vaccine; Classical swine fever virus; Porcine epidemic diarrhoea virus; Neutralising antibodies; EFFICACY; IMMUNITY;

D O I：

10.1016/j.vaccine.2021.06.084

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Many Chinese breeding pigs are repeatedly vaccinated against classical swine fever virus (CSFV) and porcine epidemic diarrhea virus (PEDV), which cause fatal, highly contagious diseases. To reduce their high frequency vaccination-induced immune stress, we constructed a combined vaccine based on the E2 protein of CSFV and the S1 spike protein subunit of PEDV (named E2-S1). In mice, the E2-S1 vaccine elicited higher neutralizing antibody titers and IgG1/IgG2a ratios against CSFV and PEDV than those induced by individual E2 or S1 vaccines. Moreover, it elicited high IL-4 expression, but no IFN-gamma expression. The results suggest that good compatibility exists between E2 and S1 antigens, and the E2-S1 vaccine can elicit a strong Th2-type cell-mediated humoral immune response. The E2-S1 recombinant fusion protein provides a novel vaccine candidate against both CSFV and PEDV, laying the foundation for future combination vaccines against swine diseases. (C) 2021 Elsevier Ltd. All rights reserved.

引用

页码：4573 / 4576

页数：4

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