Enhanced initial proliferation and differentiation of MC3T3-E1 cells on HF/HNO3 solution treated nanostructural titanium surface

被引：14

作者：

He, Fuming ^{[2
]}

Zhang, Feng ^{[1
]}

Yang, Guoli ^{[2
]}

Wang, Xiaoxiang ^{[3
]}

Zhao, Shifang ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Dept Oral & Maxillofacial Surg, Affiliated Stomatol Hosp, Hangzhou 310003, Zhejiang, Peoples R China

[2] Zhejiang Univ, Sch Med, Dept Implantol, Affiliated Stomatol Hosp, Hangzhou 310003, Zhejiang, Peoples R China

[3] Zhejiang Univ, Dept Mat Sci & Engn, Hangzhou 310003, Zhejiang, Peoples R China

来源：

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY | 2010年 / 110卷 / 04期

关键词：

OSTEOBLAST-LIKE CELLS; HYDROFLUORIC-ACID TREATMENT; IN-VIVO; GENE-EXPRESSION; DENTAL IMPLANTS; MINIATURE PIGS; BONE; VITRO; OSSEOINTEGRATION; MICROTOPOGRAPHY;

D O I：

10.1016/j.tripleo.2010.03.044

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objective. The aim of this study was to investigate the effects of nano- or submicro topography and fluoride ion on the biology of osteoblasts. Study design. Pure Ti plates were sandblasted, etched with an HCl/H2SO4 solution (control surface) and then etched in a diluted HF/HNO3 solution (test surface). MC3T3-E1 cells attached, spread, and proliferated on both surfaces. Results. The alkaline phosphatase activity was evidently higher for the test surface than for control surface after 4 and 7 days of cell culture. Real-time PCR showed significant increases in type I collagen and osteocalcin gene expression in osteoblast growth on the test surface after 4 days of culture compared with the control surface. Conclusion. With nanotopography and fluoride, hydrogen ions might improve MC3T3-E1 cell proliferation and differentiation during the early stages of cell culture. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;110:e13-e22)

引用

页码：E13 / E22

页数：10

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