Expression of normal cellular prion protein (PrPc) on T lymphocytes and the effect of copper ion:: analysis by wild-type and prion protein gene-deficient mice

被引：17

作者：

Kubosaki, A

Nishimura-Nasu, Y

Nishimura, T

Yusa, S

Sakudo, A

Saeki, K

Matsumoto, Y

Itohara, S

Onodera, T ^{[1
]}

机构：

[1] Univ Tokyo, Sch Agr & Life Sci, Dept Mol Immunol, Bunkyo Ku, Tokyo 1138657, Japan

[2] RIKEN, Brain Sci Inst, Lab Behav Genet, Wako, Saitama 3510198, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 307卷 / 04期

关键词：

prion protein; gene targeting; copper ion; interleukin-2; flow cytometry;

D O I：

10.1016/S0006-291X(03)01263-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purpose of this report was to determine the effect of prion protein (PrP) gene disruption on T lymphocyte function. Previous studies have suggested that normal cellular prion protein (PrPc) binds to copper and Cu2+ is essential for interleukin-2 (IL-2) mRNA synthesis. In this study, IL-2 mRNA levels in a copper-deficient condition were investigated using T lymphocytes from prion protein gene-deficient (PrP0/0) and wild-type mice. Results showed that Cu2+ deficiency had no effect on PrPc expression in Con A-activated splenocytes. However, a delay in IL-2 gene expression was observed in PrP0/0 mouse T lymphocyte cultures using Con A and Cu2+-chelator. These results suggest that PrPc expression may play an important role in rapid Cu2+ transfer in T lymphocytes. The rapid transfer of Cu2+ in murine T lymphocytes could be one of the normal functions of PrPc. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：810 / 813

页数：4

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