Expression of normal cellular prion protein (PrPc) on T lymphocytes and the effect of copper ion:: analysis by wild-type and prion protein gene-deficient mice

被引：17
|
作者：
Kubosaki, A
Nishimura-Nasu, Y
Nishimura, T
Yusa, S
Sakudo, A
Saeki, K
Matsumoto, Y
Itohara, S
Onodera, T [1 ]
机构：
[1] Univ Tokyo, Sch Agr & Life Sci, Dept Mol Immunol, Bunkyo Ku, Tokyo 1138657, Japan
[2] RIKEN, Brain Sci Inst, Lab Behav Genet, Wako, Saitama 3510198, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 307卷 / 04期
关键词：
prion protein; gene targeting; copper ion; interleukin-2; flow cytometry;
D O I：
10.1016/S0006-291X(03)01263-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The purpose of this report was to determine the effect of prion protein (PrP) gene disruption on T lymphocyte function. Previous studies have suggested that normal cellular prion protein (PrPc) binds to copper and Cu2+ is essential for interleukin-2 (IL-2) mRNA synthesis. In this study, IL-2 mRNA levels in a copper-deficient condition were investigated using T lymphocytes from prion protein gene-deficient (PrP0/0) and wild-type mice. Results showed that Cu2+ deficiency had no effect on PrPc expression in Con A-activated splenocytes. However, a delay in IL-2 gene expression was observed in PrP0/0 mouse T lymphocyte cultures using Con A and Cu2+-chelator. These results suggest that PrPc expression may play an important role in rapid Cu2+ transfer in T lymphocytes. The rapid transfer of Cu2+ in murine T lymphocytes could be one of the normal functions of PrPc. (C) 2003 Elsevier Inc. All rights reserved.
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收藏
页码：810 / 813
页数：4
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