Merging of Confocal and Caging Technologies: Selective Three-Color Communication with Profluorescent Reporters

被引：22

作者：

Priestman, Melanie A. ^{[1
,2
]}

Shell, Thomas A. ^{[1
,2
]}

Sun, Liang ^{[4
]}

Lee, Hsien-Ming ^{[3
]}

Lawrence, David S. ^{[1
,2
]}

机构：

[1] Univ N Carolina, Dept Chem, Div Chem Biol & Med Chem, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Dept Pharmacol, Chapel Hill, NC 27599 USA

[3] Acad Sinica, Inst Chem, Taipei 115, Taiwan

[4] Pharmaron Beijing BDA, Beijing 100176, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 31期

关键词：

caged probes; cobalamines; fluorescent probes; fluorescence spectroscopy; photolysis; PHOTOLYSIS; ALKYLCOBALAMINS; FLUORESCENCE; ACTIVATION; RELEASE; SPECTRA;

D O I：

10.1002/anie.201202820

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Falling apart, on cue: Signaling pathways often display a profound spatiotemporal component that is best studied using light-activatable reagents. Three separate photolabile moieties that can be distinguished based upon their response to three distinct wavelengths (360, 440, and 560 nm) have been synthesized and evaluated. This tri-color system is also applied to imaging in microwells and HeLa cells (see picture). Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：7684 / 7687

页数：4

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