Genome-wide identification and comparative analysis of resistance genes in Brassica juncea

被引：14

作者：

Inturrisi, Fabian ^{[1
]}

Bayer, Philipp E. ^{[1
]}

Yang, Hua ^{[1
,2
]}

Tirnaz, Soodeh ^{[1
]}

Edwards, David ^{[1
]}

Batley, Jacqueline ^{[1
]}

机构：

[1] Univ Western Australia, Sch Biol Sci, Perth, WA, Australia

[2] Univ Queensland, Sch Agr & Food Sci, Brisbane, Qld, Australia

来源：

MOLECULAR BREEDING | 2020年 / 40卷 / 08期

基金：

澳大利亚研究理事会;

关键词：

Nucleotide-binding site Leucine-rich repeat (NLR); Toll; interleukin-1 receptor (TIR) domain; Coiled-coil (CC) domain; Effector-triggered immunity (ETI); Gene cluster; Gene duplication; NBS-LRR GENES; TIR-NBS; DYNAMIC EVOLUTION; MILDEW RESISTANCE; ENCODING GENES; PLANT; DOMAINS; PROTEINS; DIVERSIFICATION; PREDICTION;

D O I：

10.1007/s11032-020-01159-z

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Disease resistance inBrassica junceahas been shown against several diseases including blackleg, white rust, hypocotyl rot, turnip mosaic virus, leaf blight, black rot, and Alternaria blight. The identification of disease resistance genes is important to develop elite cultivars, which can survive exposure to these diseases. The largest family of resistance genes are nucleotide-binding site leucine-rich repeat genes (NLRs) which are classified into two major subfamilies: toll/interleukin-1 receptor-NLR (TNL) and coiled-coil-NLR (CNL) proteins. Here we present the first study of the genomic organization and evolutionary history of the NLR gene family inB. juncea, with comparative analysis to the diploid progenitorsB. rapaandB. nigra. A total of 289 NLR genes were identified inB. juncea, with a ratio of 0.61:1 of TNL to non-TNL genes. The distribution of NLR genes was random and uneven, with chromosome A04 containing no NLR genes. Domain structure analysis revealed that 24% of NLR genes are typical resistance genes containing all three domains (TIR/CC, NBS, LRR), whereas the remaining genes are partially deleted or truncated. A total of 45% NLR genes were found to be physically clustered in theB. junceagenome. The NLR genes were analysed with OrthoFinder which showed that most physical clusters (63%) inB. junceacame from the same orthogroup. This study provides a valuable resource for the identification and characterization of candidate NLR genes for Brassica crop improvement.

引用

页数：14

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