Specific immunomodulatory and secretory activities of stevioside and steviol in intestinal cells

被引：65

作者：

Boonkaewwan, Chaiwat ^{[1
,3
]}

Ao, Mei ^{[1
]}

Toskulkao, Chaivat ^{[2
,3
]}

Rao, Mrinalini C. ^{[1
]}

机构：

[1] Univ Illinois, Dept Physiol & Biophys, Chicago, IL 60612 USA

[2] Minist Sci & Technol, Off Permanent Secretary, Bangkok 10400, Thailand

[3] Mahidol Univ, Fac Sci, Dept Physiol, Bangkok 10400, Thailand

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2008年 / 56卷 / 10期

关键词：

stevioside; steviol; IL-8; immunomodulation; Cl-; secretion; intestinal epithelial cell lines;

D O I：

10.1021/jf072681o

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Stevioside, isolated from Stevia rebaudiana, is a commercial sweetener. It was previously demonstrated that stevioside attenuates NF-kappa B-dependent TNF-alpha and IL-10 synthesis in LPS-stimulated monocytes. The present study examined the effects of stevioside and its metabolite, steviol, on human colon carcinoma cell lines. High concentrations of stevioside (2-5 mM) and steviol (0.2-0.8 mM) decreased cell viability in T84, Caco-2, and HT29 cells. Stevioside (2 mM) potentiated TNF-alpha-mediated IL-8 release in T84 cells. However, steviol (0.01-0.2 mM) significantly suppressed TNF-alpha-induced IL-8 release in all three cell lines. In T84 cells, steviol attenuated TNF-alpha-stimulated I kappa B --> NF-kappa B signaling. Chloride transport was stimulated by steviol (0.1 mM) > stevioside (1 mM) at 30 min. Two biological effects of steviol in the colon are demonstrated for the first time: stimulation of Cl- secretion and attenuation of TNF-alpha-stimulated IL-8 production. The immunomodulatory effects of steviol appear to involve NF-kappa B signaling. In contrast, at nontoxic concentrations stevioside affects only Cl- secretion.

引用

页码：3777 / 3784

页数：8

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