Biosynthesis, characterization and antimicrobial activity of silver nanoparticles by a halotolerant Bacillus endophyticus SCU-L

被引：49

作者：

Gan, Longzhan ^{[1
,2
]}

Zhang, Shuai ^{[1
,2
,3
]}

Zhang, Yi ^{[4
]}

He, Shuang ^{[1
,2
]}

Tian, Yongqiang ^{[1
,2
]}

机构：

[1] Sichuan Univ, Minist Educ, Key Lab Leather Chem & Engn, Chengdu, Sichuan, Peoples R China

[2] Sichuan Univ, Coll Light Ind Text & Food Engn, Chengdu, Sichuan, Peoples R China

[3] Sichuan Acad Food & Fermentat Ind, Chengdu, Sichuan, Peoples R China

[4] Sichuan Univ Sci & Engn, Coll Bioengn, Zigong, Peoples R China

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2018年 / 48卷 / 07期

关键词：

Antimicrobial activity; Bacillus endophyticus; biosynthesis; halotolerant bacterium; silver nanoparticles; GOLD NANOPARTICLES; BIOLOGICAL SYNTHESIS; BIOGENIC SYNTHESIS; EXTRACTS; STRAIN; FUNGUS;

D O I：

10.1080/10826068.2018.1476880

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have conducted a thorough study on extracellular biosynthesis of silver nanoparticles (AgNPs) by a halotolerant bacterium Bacillus endophyticus SCU-L, which was identified by 16S rRNA gene sequencing analysis. This strain was selected during an ongoing research programme aimed at finding a novel biological method for green nanosynthetic routes using the extremophiles in unexplored hypersaline habitats. The biosynthesized AgNPs were characterized and analyzed with UV-vis spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, atomic force microscopy and X-ray diffraction. Further, the AgNPs were found to be spherical in shape with an average particle size of about 5.1nm, and it was stable in aqueous solution for three months period of storage at room temperature under dark condition. Also, the synthesized AgNPs significantly presented antimicrobial activity against Candida albicans, Escherichia coli, Salmonella typhi and Staphylococcus aureus. The above results suggested that the present work may provide a valuable reference and theoretical basis for further exploration on microbial biosynthesis of AgNPs by halotolerant bacteria.

引用

页码：582 / 588

页数：7

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