PEG and nnPEG-Anthracene Induce DNA Condensation and Particle Formation

被引：21
作者：
Froehlich, E. [1 ]
Mandeville, J. S. [1 ]
Arnold, D. [2 ]
Kreplak, L. [2 ]
Tajmir-Riahi, H. A. [1 ]
机构：
[1] Univ Quebec Trois Rivieres, Dept Biol Chem, Trois Rivieres, PQ G9A 5H7, Canada
[2] Dalhousie Univ, Dept Phys, Halifax, NS B3H 3J5, Canada
来源：
JOURNAL OF PHYSICAL CHEMISTRY B | 2011年 / 115卷 / 32期
基金：
加拿大自然科学与工程研究理事会;
关键词：
BOVINE SERUM-ALBUMIN; STRUCTURAL-ANALYSIS; POLYETHYLENE-GLYCOL; CATIONIC LIPIDS; GENE DELIVERY; DENDRIMERS; PRECIPITATION; EFFICIENCY; COMPLEXES; IR;
D O I：
10.1021/jp205079u
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
In this study, we investigated the binding of DNA with poly(ethylene glycol) (PEG) of different sizes and compositions such as PEG 3350, PEG 6000, and mPEG-anthracene in aqueous solution at physiological conditions. The effects of size and composition on DNA aggregation and condensation as well as conformation were determined using Fourier transform infrared (FTIR), UV-visible, CD, fluorescence spectroscopic methods and atomic force microscopy (AFM). Structural analysis showed moderate complex formation for PEG 3350 and PEG 6000 and weaker interaction for mPE-anthracene-DNA adducts with both hydrophilic and hydrophobic contacts. The order of +/- stability of the complexes formed is K-PEG (6000) = 1.5 (+/- 0.4) x 10(4) M-1 > K-PEG 3350 = 7.9 (+/- 1) x 10(3) M-1 > KmPEG-anthracene = 3.6 (+/- 0.8) x 10(3) M-1 with nearly 1 bound PEG molecule per DNA. No B-DNA conformational changes were observed, while DNA condensation and particle formation occurred at high PEG concentration.
引用
收藏
页码：9873 / 9879
页数：7
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