NanoSMGT: transfection of exogenous DNA on sex-sorted bovine sperm using nanopolymer

被引：29

作者：

Campos, V. F. ^{[1
]}

Komninou, E. R. ^{[1
]}

Urtiaga, G. ^{[1
]}

de Leon, P. M. ^{[1
]}

Seixas, F. K. ^{[2
]}

Dellagostin, O. A. ^{[3
]}

Deschamps, J. C. ^{[1
]}

Collares, T. ^{[1
]}

机构：

[1] Univ Fed Pelotas, Ctr Desenvolvimento Tecnol, Lab Embriol Mol & Transgenese, Nucleo Biotecnol, Pelotas, RS, Brazil

[2] Univ Fed Pelotas, Ctr Desenvolvimento Tecnol, Lab Genom Func, Nucleo Biotecnol, Pelotas, RS, Brazil

[3] Univ Fed Pelotas, Ctr Desenvolvimento Tecnol, Mol Biol Lab, Nucleo Biotecnol, Pelotas, RS, Brazil

来源：

THERIOGENOLOGY | 2011年 / 75卷 / 08期

关键词：

NanoSMGT; Nanopolymer; Sperm-mediated gene transfer; Sexed sperm; Cattle; MEDIATED GENE-TRANSFER; BOAR SPERMATOZOA; FOREIGN DNA; DELIVERY; CELLS; BINDING; NANOPARTICLES; TRANSGENESIS; TRANSMISSION; EXPRESSION;

D O I：

10.1016/j.theriogenology.2011.01.009

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The objective was to introduce exogenous DNA into commercially sex-sorted bovine sperm using nanopolymer for transfection. In the first experiment, the optimal concentration and ratio of linear-to-circular plasmid was determined for NanoSMGT in unsorted sperm. A second experiment was conducted to transfect exogenous DNA into sex-sorted sperm. Exogenous DNA uptake occurred in a dose-dependent manner (P < 0.05). The optimal amount of DNA was 10 mu g/10(6) cells. The ratios of linear-to-circular plasmid do not influence the uptake by unsorted sperm cells and none of the tested treatments affected sperm motility and viability. Commercially sex-sorted bovine sperm were able to uptake exogenous DNA using nanopolymer; however, both X- and Y-sorted sperm had decreased DNA uptake in comparison to unsorted sperm (P < 0.05). Neither sperm motility nor viability were affected by nanotransfection. In conclusion, nanopolymer efficiently introduced exogenous DNA into commercially sex-sorted bovine sperm; we inferred that these sperm could be used for production of embryos of the desired sex, a technique named NanoSMGT. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：1476 / 1481

页数：6

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