Comparison of Commercial ELISA Blood Tests for Early Pregnancy Detection in Dairy Cows

被引：32

作者：

Piechotta, Marion ^{[1
]}

Bollwein, Julia ^{[1
]}

Friedrich, Morten ^{[2
]}

Heilkenbrinker, Thomas ^{[3
]}

Passavant, Chuck ^{[4
]}

Branen, Josh ^{[4
]}

Sasser, Garth ^{[4
]}

Hoedemaker, Martina ^{[1
]}

Bollwein, Heinrich ^{[1
]}

机构：

[1] Univ Vet Med Hannover, Clin Cattle, D-30173 Hannover, Germany

[2] Univ Gottingen, Dept Anim Sci, D-37075 Gottingen, Germany

[3] Chamber Agr Lower Saxony, Dept Anim Hlth, D-26121 Oldenburg, Germany

[4] BioTracking LLC, Moscow, ID 83843 USA

来源：

JOURNAL OF REPRODUCTION AND DEVELOPMENT | 2011年 / 57卷 / 01期

关键词：

Dairy cattle; Pregnancy-associated glycoprotein (PAG); Pregnancy-specific protein-B (PSPB); BOVINE PLASMA; PROTEIN-B; DIAGNOSIS; CATTLE; RADIOIMMUNOASSAY; GLYCOPROTEIN; SERUM; ULTRASONOGRAPHY; UTERUS; FETAL;

D O I：

10.1262/jrd.10-022T

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The objective of the present study was to compare two commercially available blood-based pregnancy tests, namely BioPRYN, an ELISA for pregnancy-specific protein B (PSPB), and an ELISA for pregnancy-associated glycoprotein (PAG), for early pregnancy diagnosis in dairy cattle using transrectal ultrasonography as a gold standard. Transrectal ultrasonography was conducted 26-58 days after artificial insemination (AI) in 197 cattle from 19 farms. Concurrently, a blood sample was collected for determination of serum PSPB and PAG. Transrectal palpation was performed approximately 120 days after Alto verify that pregnancy was maintained. For PSPB and PAG, there were no significant differences (P>0.05) in sensitivity (98.0 and 97.8%), specificity (97.1 and 91.2%), positive predictive values (99.3 and 97.8%), negative predictive values (91.9 and 91.2%) and accuracy (97.8 and 96.4%). In conclusion, the two blood pregnancy assays were equally efficacious and were highly accurate (based on transrectal ultrasonography as the gold standard).

引用

页码：72 / 75

页数：4

共 20 条

[1] Comparison of pregnancy diagnosis in dairy cattle by use of a commercial ELISA and palpation per rectum
Breed, Matthew W.
Guard, Charles L.
White, Maurice E.
Smith, Mary C.
Warnick, Lorin D.
[J]. JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION, 2009, 235 (03): : 292 - 298
[2] DETECTION AND PARTIAL CHARACTERIZATION OF 2 BOVINE PREGNANCY-SPECIFIC PROTEINS
BUTLER, JE
HAMILTON, WC
SASSER, RG
RUDER, CA
HASS, GM
WILLIAMS, RJ
[J]. BIOLOGY OF REPRODUCTION, 1982, 26 (05) : 925 - 933
[3] ASSESSMENT OF FETAL NUMBER, AND FETAL AND PLACENTAL VIABILITY THROUGHOUT PREGNANCY IN CATTLE
DOBSON, H
ROWAN, TG
KIPPAX, IS
HUMBLOT, P
[J]. THERIOGENOLOGY, 1993, 40 (02) : 411 - 425
[4] Eckblad W.P., 1985, Proc. Am. Soc. Anim. Sci. West Sec, V36, P396
[5] Establishment of an ELISA for Measuring Bovine Pregnancy-Associated Glycoprotein in Serum or Milk and Its Application for Early Pregnancy Detection
Friedrich, M.
Holtz, W.
[J]. REPRODUCTION IN DOMESTIC ANIMALS, 2010, 45 (01) : 142 - 146
[6] Early detection of pregnancy and embryonic loss in dairy cattle by ELISA tests
Gabor, G.
Torth, F.
Ozsvari, L.
Abonyi-Torth, Zs
Sasser, R. G.
[J]. REPRODUCTION IN DOMESTIC ANIMALS, 2007, 42 (06) : 633 - 636
[7] Howard J., 2007, Proc. West. Sect. Am. Soc. Anim. Sci, V58, P295
[8] HUMBLOT P, 1988, J REPROD FERTIL, V83, P215, DOI 10.1530/jrf.0.0830215
[9] DIAGNOSIS OF PREGNANCY BY RADIOIMMUNOASSAY OF A PREGNANCY-SPECIFIC PROTEIN IN THE PLASMA OF DAIRY-COWS
HUMBLOT, P
CAMOUS, S
MARTAL, J
CHARLERY, J
JEANGUYOT, N
THIBIER, M
SASSER, G
[J]. THERIOGENOLOGY, 1988, 30 (02) : 257 - 267
[10] Critical evaluation of scientific articles and other sources of information: An introduction to evidence-based veterinary medicine
Kastelic, J. P.
[J]. THERIOGENOLOGY, 2006, 66 (03) : 534 - 542

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