Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle

被引：88

作者：

Uezumi, Akiyoshi ^{[1
]}

Nakatani, Masashi ^{[1
]}

Ikemoto-Uezumi, Madoka ^{[2
]}

Yamamoto, Naoki ^{[3
]}

Morita, Mitsuhiro ^{[4
]}

Yamaguchi, Asami ^{[4
]}

Yamada, Harumoto ^{[4
]}

Kasai, Takehiro ^{[5
]}

Masuda, Satoru ^{[6
]}

Narita, Asako ^{[6
]}

Miyagoe-Suzuki, Yuko ^{[6
]}

Takeda, Shin'ichi ^{[6
]}

Fukada, So-ichiro ^{[7
]}

Nishino, Ichizo ^{[8
]}

Tsuchida, Kunihiro ^{[1
]}

机构：

[1] Fujita Hlth Univ, Inst Comprehens Med Sci, Div Therapies Intractable Dis, 1-98 Dengakugakubo, Toyoake, Aichi 4701192, Japan

[2] Natl Ctr Geriatr & Gerontol, Natl Inst Longev Sci, Dept Regenerat Med, 35 Gengo, Obu, Aichi 4748511, Japan

[3] Fujita Hlth Univ, Lab Mol Biol & Histochem, Toyoake, Aichi 4701192, Japan

[4] Fujita Hlth Univ, Dept Orthopaed Surg, Toyoake, Aichi 4701192, Japan

[5] Nagoya Univ, Grad Sch Med, Dept Orthoped Surg, Showa Ku, 65 Tsurumai, Nagoya, Aichi 4668550, Japan

[6] Natl Ctr Neurol & Psychiat, Natl Inst Neurosci, Dept Mol Therapy, 4-1-1 Ogawa Higashi, Kodaira, Tokyo 1878502, Japan

[7] Osaka Univ, Grad Sch Pharmaceut Sci, Dept Immunol, 1-6 Yamada Oka, Suita, Osaka 5650871, Japan

[8] Natl Ctr Neurol & Psychiat, Dept Neuromuscular Res, Natl Inst Neurosci, 4-1-1 Ogawa Higashi, Kodaira, Tokyo 1878502, Japan

来源：

STEM CELL REPORTS | 2016年 / 7卷 / 02期

关键词：

HEMATOPOIETIC STEM-CELLS; SATELLITE CELLS; MESENCHYMAL PROGENITORS; METASTASIS SUPPRESSOR; FIBRO/ADIPOGENIC PROGENITORS; HETEROTOPIC OSSIFICATION; HUMAN FIBROBLASTS; C RECEPTOR; IN-VITRO; EXPRESSION;

D O I：

10.1016/j.stemcr.2016.07.004

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Skeletal muscle contains two distinct stem/progenitor populations. One is the satellite cell, which acts as a muscle stem cell, and the other is the mesenchymal progenitor, which contributes to muscle pathogeneses such as fat infiltration and fibrosis. Detailed and accurate characterization of these progenitors in humans remains elusive. Here, we performed comprehensive cell-surface protein profiling of the two progenitor populations residing in human skeletal muscle and identified three previously unrecognized markers: CD82 and CD318 for satellite cells and CD201 for mesenchymal progenitors. These markers distinguish myogenic and mesenchymal progenitors, and enable efficient isolation of the two types of progenitors. Functional study revealed that CD82 ensures expansion and preservation of myogenic progenitors by suppressing excessive differentiation, and CD201 signaling favors adipogenesis of mesenchymal progenitors. Thus, cell-surface proteins identified here are not only useful markers but also functionally important molecules, and provide valuable insight into human muscle biology and diseases.

引用

页码：263 / 278

页数：16

共 45 条

[1] Human skeletal muscle fibroblasts, but not myogenic cells, readily undergo adipogenic differentiation [J].