Membrane Properties of Human Induced Pluripotent Stem Cell-Derived Cultured Red Blood Cells

被引：8

作者：

Bernecker, Claudia ^{[1
]}

Matzhold, Eva Maria ^{[1
]}

Kolb, Dagmar ^{[2
,3
]}

Avdili, Afrim ^{[1
]}

Rohrhofer, Lisa ^{[1
]}

Lampl, Annika ^{[1
]}

Troetzmueller, Martin ^{[4
]}

Singer, Heike ^{[5
]}

Oldenburg, Johannes ^{[5
]}

Schlenke, Peter ^{[1
]}

Dorn, Isabel ^{[1
]}

机构：

[1] Med Univ Graz, Dept Blood Grp Serol & Transfus Med, A-8036 Graz, Austria

[2] Med Univ Graz, Core Facil Ultrastruct Anal, A-8010 Graz, Austria

[3] Med Univ Graz, Gottfried Schatz Res Ctr Cell Signaling Metab & A, Div Cell Biol Histol & Embryol, A-8010 Graz, Austria

[4] Med Univ Graz, Ctr Med Res, Core Facil Mass Spectrometry, A-8010 Graz, Austria

[5] Univ Clin Bonn, Inst Expt Haematol & Transfus Med, D-53127 Bonn, Germany

来源：

CELLS | 2022年 / 11卷 / 16期

关键词：

red blood cells; induced pluripotent stem cells; erythropoiesis; membrane; blood group antigen; deformability; osmotic resistance; phospholipids; cholesterol; reticulocytes; GENERATION; DIFFERENTIATION; ERYTHROPOIESIS; RETICULOCYTES; MITOCHONDRIA; MATURATION; PLATFORM; PLASMA; BAND-3;

D O I：

10.3390/cells11162473

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cultured red blood cells from human induced pluripotent stem cells (cRBC_iPSCs) are a promising source for future concepts in transfusion medicine. Before cRBC_iPSCs will have entrance into clinical or laboratory use, their functional properties and safety have to be carefully validated. Due to the limitations of established culture systems, such studies are still missing. Improved erythropoiesis in a recently established culture system, closer simulating the physiological niche, enabled us to conduct functional characterization of enucleated cRBC_iPSCs with a focus on membrane properties. Morphology and maturation stage of cRBC_iPSCs were closer to native reticulocytes (nRETs) than to native red blood cells (nRBCs). Whereas osmotic resistance of cRBC_iPSCs was similar to nRETs, their deformability was slightly impaired. Since no obvious alterations in membrane morphology, lipid composition, and major membrane associated protein patterns were observed, reduced deformability might be caused by a more primitive nature of cRBC_iPSCs comparable to human embryonic- or fetal liver erythropoiesis. Blood group phenotyping of cRBC_iPSCs further confirmed the potency of cRBC_iPSCs as a prospective device in pre-transfusional routine diagnostics. Therefore, RBC membrane analyses obtained in this study underscore the overall prospects of cRBC_iPSCs for their future application in the field of transfusion medicine.

引用

页数：20

共 55 条

[1] The aging population poses a global challenge for blood services [J].