Metabolomic strategy to study therapeutic and synergistic effects of tanshinone IIA, salvianolic acid B and ginsenoside Rb1 in myocardial ischemia rats

被引：32
作者：
Lu, Yonghai [1 ]
Liu, Xinru [1 ]
Liang, Xu [1 ]
Xiang, Li [1 ]
Zhang, Weidong [1 ,2 ]
机构：
[1] Second Mil Med Univ, Sch Pharm, Dept Med Chem Nat Prod, Shanghai 200433, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200030, Peoples R China
来源：
JOURNAL OF ETHNOPHARMACOLOGY | 2011年 / 134卷 / 01期
关键词：
Tanshinone IIA (T); Salvianolic acid B (S); Ginsenoside Rb1 (G); Therapeutic effect; Synergistic effect; Metabolomics; FLIGHT MASS-SPECTROMETRY; DANSHEN TABLETS; METABONOMICS; REPERFUSION; INFARCTION; INTERVENTION; CONSTITUENTS; PROTECTS; INJURY; SERUM;
D O I：
10.1016/j.jep.2010.11.048
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Aim of the study: Tanshinone IIA (T), salvianolic acid B(S) and ginsenoside Rb1 (G) are the three major active ingredients of Compound Danshen Formula (CDF) for its protective effects on myocardial ischemia (MI). In this study, we aimed to investigate therapeutic and synergistic effects of TSG (combination of T. S and G) on MI rats with metabolomic strategy. Materials and methods: MI model were induced in Sprague-Dawley rats by left anterior descending coronary artery ligation. MI rats were respectively administrated T, S, G, TSG and CDF. Plasma was analyzed by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Partial least squares discriminate analysis (PLS-DA) models were built to evaluate the therapeutic and synergistic effects of TSG at whole level. 22 MI biomarkers in rat plasma were also investigated to explain that. Results: TSG brings nearly equal therapeutic effects on MI as CDF and it plays more stable regulated action on those 22 identified metabolites than single compound. Conclusions: Overall, there were few methods for the study of synergistic effects of Chinese medicine. Our results suggested that metabolomics offers a new idea for Chinese medicine research. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：45 / 49
页数：5
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