Glutamate production from β-glucan using endoglucanase-secreting Corynebacterium glutamicum

被引：32

作者：

Tsuchidate, Takeyuki ^{[1
]}

Tateno, Toshihiro ^{[2
]}

Okai, Naoko ^{[3
]}

Tanaka, Tsutomu ^{[3
]}

Ogino, Chiaki ^{[1
]}

Kondo, Akihiko ^{[1
]}

机构：

[1] Kobe Univ, Dept Chem Sci & Engn, Grad Sch Engn, Nada Ku, Kobe, Hyogo 6578501, Japan

[2] Kobe Univ, Dept Mol Sci & Mat Engn, Grad Sch Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan

[3] Kobe Univ, Org Adv Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2011年 / 90卷 / 03期

关键词：

Corynebacterium glutamicum; Endoglucanase; Signal sequence; Glutamate fermentation; beta-Glucan; BREVIBACTERIUM-LACTOFERMENTUM; PROTEIN EXPORT; ALPHA-AMYLASE; AMINO-ACIDS; BACTERIA; ETHANOL; STRAIN; FERMENTATION; EXPRESSION; CADAVERINE;

D O I：

10.1007/s00253-011-3116-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We demonstrate glutamate production from beta-glucan using endoglucanase (EG)-expressing Corynebacterium glutamicum. The signal sequence torA derived from Escherichia coli K12, which belongs to the Tat pathway, was suitable for secreting EG of Clostridium thermocellum using C. glutamicum as a host. Using the torA signal sequence, endoglucanase from Clostridium cellulovorans 743B was successfully expressed, and the secreted EG produced 123 mg of reducing sugar from 5 g of beta-glucan at 30 A degrees C for 72 h, which is the optimal condition for C. glutamicum growth. Subsequently, glutamate fermentation from beta-glucan was carried out with the addition of Aspergillus aculeatus beta-glucosidase produced by recombinant Aspergillus oryzae. Using EG-secreting C. glutamicum, 178 mg/l of glutamate was produced from 15 g of beta-glucan. This is the first report of glutamate fermentation from beta-glucan using endoglucanase-secreting C. glutamicum.

引用

页码：895 / 901

页数：7

共 27 条

[1] Construction of an Aspergillus oryzae cell-surface display system using a putative GPI-anchored protein [J].

Adachi, Takashi ;

Ito, Junji ;

Kawata, Kouji ;

Kaya, Masahiro ;

Ishida, Hiroki ;

Sahara, Hiroshi ;

Hata, Yoji ;

Ogino, Chiaki ;

Fukuda, Hideki ;

Kondo, Akihiko .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 81 (04) :711-719

[2] Expression of the genes coding for the xylanase Xys1 and the cellulase Cel1 from the straw-decomposing Streptomyces halstedii JM8 cloned into the amino-acid producer Brevibacterium lactofermentum ATCC13869 [J].

Adham, SAI ;

Honrubia, P ;

Díaz, M ;

Fernández-Abalos, JM ;

Santamaría, RI ;

Gil, JA .

ARCHIVES OF MICROBIOLOGY, 2001, 177 (01) :91-97

[3] The Tat protein export pathway [J].

Berks, BC ;

Sargent, F ;

Palmer, T .

MOLECULAR MICROBIOLOGY, 2000, 35 (02) :260-274

[4] Polymers derived from the amino acid L-tyrosine: polycarbonates, polyarylates and copolymers with poly(ethylene glycol) [J].

Bourke, SL ;

Kohn, J .

ADVANCED DRUG DELIVERY REVIEWS, 2003, 55 (04) :447-466

[5] Production of native-type Streptoverticillium mobaraense transglutaminase in Corynebactetium glutamicum [J].

Date, M ;

Yokoyama, KI ;

Umezawa, Y ;

Matsui, H ;

Kikuchi, Y .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (05) :3011-3014

[6] Synergistic saccharification, and direct fermentation to ethanol, of amorphous cellulose by use of an engineered yeast strain codisplaying three types of cellulolytic enzyme [J].

Fujita, Y ;

Ito, J ;

Ueda, M ;

Fukuda, H ;

Kondo, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (02) :1207-1212

[7] Direct and efficient production of ethanol from cellulosic material with a yeast strain displaying cellulolytic enzymes [J].

Fujita, Y ;

Takahashi, S ;

Ueda, M ;

Tanaka, A ;

Okada, H ;

Morikawa, Y ;

Kawaguchi, T ;

Arai, M ;

Fukuda, H ;

Kondo, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (10) :5136-5141

[8] Polymerization of polyfunctional macromolecules: Synthesis of a new class of high molecular weight poly(amino acid)s by oxidative coupling of phenol-containing precursor polymers [J].

Fukuoka, T ;

Uyama, H ;

Kobayashi, S .

BIOMACROMOLECULES, 2004, 5 (03) :977-983

[9]

George M.G., 2001, BERGEYS MANUAL SYSTE, V2nd

[10] Industrial production of amino acids by coryneform bacteria [J].

Hermann, T .

JOURNAL OF BIOTECHNOLOGY, 2003, 104 (1-3) :155-172

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