A novel FRET 'off-on' fluorescent probe for the selective detection of Fe3+, Al3+ and Cr3+ ions: Its ultrafast energy transfer kinetics and application in live cell imaging

被引：100

作者：

Chereddy, Narendra Reddy ^{[1
]}

Nagaraju, Peethani ^{[1
]}

Raju, M. V. Niladri ^{[1
]}

Krishnaswamy, Venkat Raghavan ^{[2
]}

Korrapati, Puma Sai ^{[2
]}

Bangal, Prakriti Ranjan ^{[3
]}

Rao, Vaidya Jayathirtha ^{[1
,4
]}

机构：

[1] CSIR Indian Inst Chem Technol, Crop Protect Chem Div, Hyderabad 500007, Andhra Pradesh, India

[2] CSIR Cent Leather Res Inst, Biomat Lab, Madras 600020, Tamil Nadu, India

[3] CSIR Indian Inst Chem Technol, Inorgan & Phys Chem Div, Hyderabad 500007, Andhra Pradesh, India

[4] CSIR Indian Inst Chem Technol, AcSIR, Hyderabad 500007, Andhra Pradesh, India

来源：

BIOSENSORS & BIOELECTRONICS | 2015年 / 68卷

关键词：

Rhodamine-naphthalimide dyad; Trivalent metal ion detection; Time resolved fluorescence; Ratiometric sensors; Live cell imaging; IRON REGULATORY PROTEINS; RATIOMETRIC DETECTION; OXIDATIVE STRESS; CHEMOSENSOR; ALUMINUM; HOMEOSTASIS; SENSOR; HG2+;

D O I：

10.1016/j.bios.2015.01.074

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A rhodamine-naphthalimide dyad probe, 1, that selectively responds to the addition of trivalent metal ions (Fe3+ or Al3+ or Cr3+) via ultrafast Forster resonance energy transfer (FRET) from naphthalimide to rhodamine is designed and synthesized. 1 is highly selective to the trivalent metal ions and the presence of other monovalent or divalent metal ions do not affect its detection ability. The probe is highly sensitive and it can respond to the presence of trivalent metal ions even at sub-micromolar levels. 1 is stable over a broad range of pH, non-toxic under experimental conditions and suitable to the fluorescence bio-imaging of live cells exposed to trivalent metal ions. The trivalent metal ion induced ultrafast energy transfer kinetics of 1 is explored using time resolved fluorescence experiments. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：749 / 756

页数：8

共 37 条

[1] Iron metabolism
Aisen, P
Wessling-Resnick, M
Leibold, EA
[J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 1999, 3 (02) : 200 - 206
[2] Medical progress: Disorders of iron metabolism
Andrews, NC
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1999, 341 (26) : 1986 - 1995
[3] A new selective fluorogenic probe for trivalent cations
Barba-Bon, Andrea
Costero, Ana M.
Gil, Salvador
Parra, Margarita
Soto, Juan
Martinez-Manez, Ramon
Sancenon, Felix
[J]. CHEMICAL COMMUNICATIONS, 2012, 48 (24) : 3000 - 3002
[4] Aluminium speciation in relation to aluminium bioavailability, metabolism and toxicity
Berthon, G
[J]. COORDINATION CHEMISTRY REVIEWS, 2002, 228 (02) : 319 - 341
[5] Iron deficiency and erythropoiesis: New diagnostic approaches
Brugnara, C
[J]. CLINICAL CHEMISTRY, 2003, 49 (10) : 1573 - 1578
[6] Fluorescent Chemosensors Based on Spiroring-Opening of Xanthenes and Related Derivatives
Chen, Xiaoqiang
Pradhan, Tuhin
Wang, Fang
Kim, Jong Seung
Yoon, Juyoung
[J]. CHEMICAL REVIEWS, 2012, 112 (03) : 1910 - 1956
[7] A pyridinyl-functionalized tetraphenylethylene fluorogen for specific sensing of trivalent cations
Chen, Xiujuan
Shen, Xiao Yuan
Guan, Erjia
Liu, Yi
Qin, Anjun
Sun, Jing Zhi
Tang, Ben Zhong
[J]. CHEMICAL COMMUNICATIONS, 2013, 49 (15) : 1503 - 1505
[8] Chereddy N.R., 2012, SENSOR ACTUAT B-CHEM, V294, P171
[9] A two fluorophore embedded probe for collective and ratiometric detection of Hg2+ and F- ions
Chereddy, Narendra Reddy
Nagaraju, Peethani
Raju, M. V. Niladri
Saranraj, Krishnan
Thennarasu, Sathiah
Rao, Vaidya Jayathirtha
[J]. DYES AND PIGMENTS, 2015, 112 : 201 - 209
[10] A highly selective and efficient single molecular FRET based sensor for ratiometric detection of Fe3+ ions
Chereddy, Narendra Reddy
Thennarasu, Sathiah
Mandal, Asit Baran
[J]. ANALYST, 2013, 138 (05) : 1334 - 1337

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