Photochromic rhodamines provide nanoscopy with optical sectioning

被引：216

作者：

Foelling, J. ^{[1
]}

Belov, V. ^{[1
]}

Kunetsky, R. ^{[1
]}

Medda, R. ^{[1
]}

Schoenle, A. ^{[1
]}

Egner, A. ^{[1
]}

Eggeling, C. ^{[1
]}

Bossi, M. ^{[1
]}

Hell, S. W. ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Abt NanoBiophoton, D-37077 Gottingen, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 33期

关键词：

dyes/pigments; fluorescence; microscopy; photochromism; photoswitchable compounds;

D O I：

10.1002/anie.200702167

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Exciting developments: Switching individual photochromic and fluorescent rhodamine amides enables 3D far-field optical microscopy with nanoscale resolution, excellent signal-to-noise ratio, and fast acquisition times. The rhodamine amides can be switched on using two photons, which enables 3D detailed imaging of thick and densely stained samples (such as 5-μm silica beads (see image) and living cells) to be constructed. (Figure Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：6266 / 6270

页数：5

共 17 条

[1]

Abbe E., 1873, ARCH MIKROSK ANAT, V9, P413, DOI [10.1007/BF02956173, DOI 10.1007/BF02956173]

[2] A maximum-likelihood formalism for sub-resolution axial localization of fluorescent nanoparticles [J].

Aguet, F ;

Van De Ville, D ;

Unser, M .

OPTICS EXPRESS, 2005, 13 (26) :10503-10522

[3] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[4] Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability [J].

Bossi, Mariano ;

Foelling, Jonas ;

Dyba, Marcus ;

Westphal, Volker ;

Hell, Stefan W. .

NEW JOURNAL OF PHYSICS, 2006, 8

[5] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].

DENK, W ;

STRICKLER, JH ;

WEBB, WW .

SCIENCE, 1990, 248 (4951) :73-76

[6] Photobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of two-step photolysis [J].

Eggeling, C ;

Widengren, J ;

Rigler, R ;

Seidel, CAM .

ANALYTICAL CHEMISTRY, 1998, 70 (13) :2651-2659

[7]

FERINGA BL, 2001, MOL SWITHCES

[8] IODIDE DEALKYLATION OF BENZYL, PMB, PNB, AND T-BUTYL N-ACYL AMINO-ACID ESTERS VIA LITHIUM ION COORDINATION [J].

FISHER, JW ;

TRINKLE, KL .

TETRAHEDRON LETTERS, 1994, 35 (16) :2505-2508

[9] Imaging and writing at the nanoscale with focused visible light through saturable optical transitions [J].

Hell, SW ;

Jakobs, S ;

Kastrup, L .

APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, 2003, 77 (07) :859-860

[10] BREAKING THE DIFFRACTION RESOLUTION LIMIT BY STIMULATED-EMISSION - STIMULATED-EMISSION-DEPLETION FLUORESCENCE MICROSCOPY [J].

HELL, SW ;

WICHMANN, J .

OPTICS LETTERS, 1994, 19 (11) :780-782

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