Dual-Sensitive Micellar Nanoparticles Regulate DNA Unpacking and Enhance Gene-Delivery Efficiency

被引：44

作者：

Jiang, Xuan ^{[1
]}

Zheng, Yiran ^{[3
]}

Chen, Hunter H. ^{[4
]}

Leong, Kam W. ^{[5
]}

Wang, Tza-Huei ^{[4
,6
]}

Mao, Hai-Quan ^{[1
,2
]}

机构：

[1] Johns Hopkins Univ, Dept Mat Sci & Engn, Baltimore, MD 21218 USA

[2] Johns Hopkins Univ, Whitaker Biomed Engn Inst, Baltimore, MD 21218 USA

[3] Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD 21218 USA

[4] Johns Hopkins Univ, Sch Med, Dept Biomed Engn, Baltimore, MD 21231 USA

[5] Duke Univ, Dept Biomed Engn, Durham, NC 27708 USA

[6] Johns Hopkins Univ, Dept Mech Engn, Baltimore, MD 21218 USA

来源：

ADVANCED MATERIALS | 2010年 / 22卷 / 23期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

NUCLEIC-ACIDS; CROSS-LINKING; IN-VIVO; DISULFIDE; POLYPLEXES; EXPRESSION; COMPLEX;

D O I：

10.1002/adma.200903933

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Reversibly crosslinked micellar nanoparticles that are capable of releasing encapsulated DNA in response to a reduction microenvironment and physiological ionic strength are synthesized for gene delivery. The dual-sensitive nanoparticles exhibit significantly enhanced complex and colloidal stability, yield more sustained DNA unpacking in cytosols, and mediate enhanced and more prolonged transgene expression for at least 10 days.

引用

页码：2556 / 2560

页数：5

共 11 条

[1] Delivery of Nucleic Acids via Disulfide-Based Carrier Systems [J].

Bauhuber, Sonja ;

Hozsa, Constantin ;

Breunig, Miriam ;

Goepferich, Achim .

ADVANCED MATERIALS, 2009, 21 (32-33) :3286-3306

[2] Quantitative comparison of intracellular unpacking kinetics of polyplexes by a model constructed from quantum Dot-FRET [J].

Chen, Hunter H. ;

Ho, Yi-Ping ;

Jiang, Xuan ;

Mao, Hai-Quan ;

Wang, Tza-Huei ;

Leong, Kam W. .

MOLECULAR THERAPY, 2008, 16 (02) :324-332

[3] Simultaneous non-invasive analysis of DNA condensation and stability by two-step QD-FRET [J].

Chen, Hunter H. ;

Ho, Yi-Ping ;

Jiang, Xuan ;

Mao, Hai-Quan ;

Wang, Tza-Huei ;

Leong, Kam W. .

NANO TODAY, 2009, 4 (02) :125-134

[4] PEG-b-PPA/DNA micelles improve transgene expression in rat liver through intrabiliary infusion [J].

Jiang, Xuan ;

Dai, Hui ;

Ke, Chyan-Ying ;

Mo, Xiao ;

Torbenson, Michael S. ;

Li, Zhiping ;

Mao, Hai-Quan .

JOURNAL OF CONTROLLED RELEASE, 2007, 122 (03) :297-304

[5] Environment-sensitive stabilization of core-shell structured polyion complex micelle by reversible cross-linking of the core through disulfide bond [J].

Kakizawa, Y ;

Harada, A ;

Kataoka, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (48) :11247-11248

[6] A FLUORESCENT COMPLEX BETWEEN ETHIDIUM BROMIDE AND NUCLEIC ACIDS - PHYSICAL-CHEMICAL CHARACTERIZATION [J].

LEPECQ, JB ;

PAOLETTI, C .

JOURNAL OF MOLECULAR BIOLOGY, 1967, 27 (01) :87-+

[7] Polyplex gene delivery modulated by redox potential gradients [J].

Manickam, Devika Soundara ;

Oupicky, David .

JOURNAL OF DRUG TARGETING, 2006, 14 (08) :519-526

[8] Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency [J].

Mao, HQ ;

Roy, K ;

Troung-Le, VL ;

Janes, KA ;

Lin, KY ;

Wang, Y ;

August, JT ;

Leong, KW .

JOURNAL OF CONTROLLED RELEASE, 2001, 70 (03) :399-421

[9] Block catiomer polyplexes with regulated densities of charge and disulfide cross-linking directed to enhance gene expression [J].

Miyata, K ;

Kakizawa, Y ;

Nishiyama, N ;

Harada, A ;

Yamasaki, Y ;

Koyama, H ;

Kataoka, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (08) :2355-2361

[10] Crosslinked nanocarriers based upon poly(ethylene imine) for systemic plasmid delivery: In vitro characterization and in vivo studies in mice [J].

Neu, Michael ;

Germershaus, Oliver ;

Mao, Shirui ;

Voigt, Karl-Heinz ;

Behe, Martin ;

Kissel, Thomas .

JOURNAL OF CONTROLLED RELEASE, 2007, 118 (03) :370-380

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