Fast and direct quantification of adrenal steroids by tandem mass spectrometry in serum and dried blood spots

被引：92

作者：

Janzen, Nils ^{[1
]}

Sander, Stefanie ^{[1
]}

Terhardt, Michael ^{[1
]}

Peter, Michael ^{[1
]}

Sander, Johannes ^{[1
]}

机构：

[1] State Screening Lab, D-30430 Hannover, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 861卷 / 01期

关键词：

steroid profile; tandem mass spectrometry; adrenal cortex disorders;

D O I：

10.1016/j.jchromb.2007.11.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a fast and reproducible method for steroid analysis (corticosterone, deoxycorticosterone, progesterone, 17 alpha-hydroxyprogesterone, 11-deoxycortisol, 21-deoxycortisol, androstenedione, testosterone, dihydrotestosterone and cortisol) in small volumes of serum and in dried blood spot samples by LC-MS/MS. No derivatisation was needed. LC separation was achieved by using an Atlantis (R) C-18 column and water-methanol-formic acid gradient as a mobile phase and a flow rate of 250 mu L/min over a run time of 6 min. Steroids were measured in MRM mode with electrospray interface (positive ion mode). Validation showed excellent precision, sensitivity, recovery and linearity with coefficients of determination r(2) > 0.992. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：117 / 122

页数：6

共 17 条

[1]

Chace D. H., 2007, CLSI, V27, P1

[2] Analysis of 21-deoxycortisol, a marker of congenital adrenal hyperplasia, in blood by atmospheric pressure chemical ionization and electrospray ionization using multiple reaction monitoring [J].