Nigella sativa Extract as a Potent Antioxidant for Petrochemical-Induced Oxidative Stress

被引:63
作者
Ashraf, S. Salman [1 ]
Rao, Madduri V. [1 ]
Kaneez, Fatima Shad [2 ]
Qadri, Shahnaz [2 ]
Al-Marzouqi, Ali H. [3 ]
Chandranath, Irwin S. [4 ]
Adem, Abdu [4 ]
机构
[1] UAE Univ, Dept Chem, Al Ain, U Arab Emirates
[2] UAE Univ, Dept Biol, Al Ain, U Arab Emirates
[3] UAE Univ, Dept Chem & Petr Engn, Al Ain, U Arab Emirates
[4] UAE Univ, Dept Pharmacol & Therapeut, Al Ain, U Arab Emirates
关键词
OIL; SFE; TOLUENE; YIELD;
D O I
10.1093/chrsci/49.4.321
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Various beneficial properties has been attributed to Nigella sativa, including its antioxidant potential. Previously, it was reported that supercritical fluid extraction (SFE) could be used to obtain N. sativa extract rich in antioxidants. In the present study, N. sativa extracts prepared using the previously optimized SFE as well as the traditional Soxhlet extraction approaches were analyzed for various known antioxidants. N. sativa extracts were found to prevent protein carbonyl formation as well as depletion of intracellular glutathione (GSH) in fibroblasts exposed to toluene. Furthermore, partially purified SFE and Soxhlet fractions could prevent loss of hepatic GSH in toluene-induced oxidative stressed Wistar rats as well as in L929 fibroblasts. The results showed that SFE-produced N. sativa extract is richer in antioxidants than the Soxhlet approach. It was also shown using preparative silica gel and reverse phase chromatography that different fractions of SFE-extracted or Soxhletextracted N. sativa had different levels of protective effects with regards to GSH depletion in vivo as well as in cell culture. Although fractions rich in thymoquinone were found to be most potent in terms of antioxidant capacity, the data indicates that the protective effects of N. sativa may not only be due to thymoquinone, but perhaps other antioxidants.
引用
收藏
页码:321 / 326
页数:6
相关论文
共 26 条
[1]   Comparative evaluation of SFE and steam distillation methods on the yield and composition of essential oil extracted from spearmint (Mentha spicata) [J].
Al-Marzouqi, Ali H. ;
Rao, Madduri V. ;
Jobe, Baboucarr .
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2007, 30 (04) :463-475
[2]   Pharmacological and toxicological properties of Nigella sativa [J].
Ali, BH ;
Blunden, G .
PHYTOTHERAPY RESEARCH, 2003, 17 (04) :299-305
[3]   Protein S-thiolation and depletion of intracellular glutathione in skin fibroblasts exposed to various sources of oxidative stress [J].
Ashraf, S. Salman ;
Galadari, Sehamuddin ;
Patel, Mahendra .
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2006, 22 (01) :75-79
[4]   Extraction of oilseeds by SFE -: a comparison with other methods for the determination of the oil content [J].
Brühl, L ;
Matthäus, B .
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1999, 364 (07) :631-634
[5]  
Burits M, 2000, PHYTOTHER RES, V14, P323, DOI 10.1002/1099-1573(200008)14:5<323::AID-PTR621>3.0.CO
[6]  
2-Q
[7]   CENTRAL ROLE OF RADICAL CATIONS IN METABOLIC-ACTIVATION OF POLYCYCLIC AROMATIC-HYDROCARBONS [J].
CAVALIERI, EL ;
ROGAN, EG .
XENOBIOTICA, 1995, 25 (07) :677-688
[8]   The effect of m-xylene on cytotoxicity and cellular antioxidant status in rat dermal equivalents [J].
Coleman, CA ;
Hull, BE ;
McDougal, JN ;
Rogers, JV .
TOXICOLOGY LETTERS, 2003, 142 (1-2) :133-142
[9]  
Dragland S, 2003, J NUTR, V133, P1286
[10]   Changes in markers of oxidative stress and membrane properties in synaptosomes from rats exposed prenatally to toluene [J].
Edelfors, S ;
Hass, U ;
Hougaard, KS .
PHARMACOLOGY & TOXICOLOGY, 2002, 90 (01) :26-31