Increasing the Antibacterial Effect of Lysozyme by Immobilization on Multi-Walled Carbon Nanotubes

被引：23

作者：

Merli, Daniele ^{[2
]}

Ugonino, Monica ^{[2
]}

Profumo, Antonella ^{[2
]}

Fagnoni, Maurizio ^{[3
]}

Quartarone, Eliana ^{[1
]}

Mustarelli, Piercarlo ^{[1
,5
]}

Visai, Livia ^{[4
,5
]}

Grandi, Marco S. ^{[6
]}

Galinetto, Pietro ^{[6
]}

Canton, Patrizia ^{[7
]}

机构：

[1] Univ Pavia, Dept Phys Chem, I-27100 Pavia, Italy

[2] Univ Pavia, Dept Gen Chem, I-27100 Pavia, Italy

[3] Univ Pavia, Dept Organ Chem, I-27100 Pavia, Italy

[4] Univ Pavia, Dept Biochem, I-27100 Pavia, Italy

[5] Univ Pavia, Ctr Tissue Engn CIT, I-27100 Pavia, Italy

[6] Univ Pavia, Dept Phys A Volta, I-27100 Pavia, Italy

[7] Univ Ca Foscari Venezia, Dipartimento Chim Fis, I-30170 Venice, Italy

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2011年 / 11卷 / 04期

关键词：

Nanomedicine; Carbon Nanotube; Lysozyme; Covalent Binding; Antibacterial Activity; PEPTIDES; FUNCTIONALIZATION; STABILITY; OXIDATION;

D O I：

10.1166/jnn.2011.3758

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a facile strategy to obtain multiwalled carbon nanotubes (MWCNTs) functionalized with covalently bonded lysozyme. The functionalization procedure has been investigated by means of several techniques, including thermogravimetry, Raman spectroscopy, transmission electron microscopy, and cyclic voltammetry. A functionalization of about 1 lysozyme molecule every 4000 carbon atoms is obtained. The modified lysozyme-CNTs nanocomposite shows a significant increase of the antibacterial activity towards the Gram-positive S. aureus if compared with lysozyme in solution.

引用

页码：3100 / 3106

页数：7

共 41 条

[1] Regulatory Peptides Are Susceptible to Oxidation by Metallic Impurities within Carbon Nanotubes [J].