DNA assembly standards: Setting the low-level programming code for plant biotechnology

被引:16
作者
Vazquez-Vilar, Marta [1 ]
Orzaez, Diego [2 ]
Patron, Nicola [3 ]
机构
[1] Wageningen Univ & Res, Lab Syst & Synthet Biol, Stippeneng 4, NL-6708 WE Wageningen, Netherlands
[2] Univ Politecn Valencia, CSIC, IBMCP, Valencia, Spain
[3] Norwich Res Pk, Earlham Inst, Dept Engn Biol, Norwich NR1 7UZ, Norfolk, England
基金
英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;
关键词
Synthetic biology; DNA assembly; Molecular cloning; Laboratory automation; Biotechnology; Genome engineering; CRISPR; SYNTHETIC BIOLOGY; GENE-EXPRESSION; DESIGN; TRANSCRIPTION; MICROFLUIDICS; INTEGRATION; BLUEPRINTS; EFFICIENT; STACKING; PLATFORM;
D O I
10.1016/j.plantsci.2018.02.024
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Synthetic Biology is defined as the application of engineering principles to biology. It aims to increase the speed, ease and predictability with which desirable changes and novel traits can be conferred to living cells. The initial steps in this process aim to simplify the encoding of new instructions in DNA by establishing low-level programming languages for biology. Together with advances in the laboratory that allow multiple DNA molecules to be efficiently assembled together into a desired order in a single step, this approach has simplified the design and assembly of multigene constructs and has even facilitated the automated construction of synthetic chromosomes. These advances and technologies are now being applied to plants, for which there are a growing number of software and wetware tools for the design, construction and delivery of DNA molecules and for the engineering of endogenous genes. Here we review the efforts of the past decade that have established synthetic biology workflows and tools for plants and discuss the constraints and bottlenecks of this emerging field.
引用
收藏
页码:33 / 41
页数:9
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