Medium optimization based on the metabolic-flux spectrum of recombinant Escherichia coli for high expression of human-like collagen II

被引：31

作者：

Guo, Jiaqing ^{[1
,2
,3
]}

Luo, Yan'e ^{[1
,2
,3
]}

Fan, Daidi ^{[1
,2
,3
]}

Yang, Bo ^{[3
]}

Gao, Pengfei ^{[1
,2
,3
]}

Ma, Xiaoxuan ^{[1
,2
,3
]}

Zhu, Chenhui ^{[1
,2
,3
]}

机构：

[1] NW Univ Xian, Shaanxi Key Lab Degradable Biomed Mat, Xian 710069, Shaanxi, Peoples R China

[2] NW Univ Xian, Shaanxi R&D Ctr Biomat & Fermentat Engn, Xian 710069, Shaanxi, Peoples R China

[3] NW Univ Xian, Sch Chem Engn, Xian 710069, Shaanxi, Peoples R China

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2010年 / 57卷

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

carbon/nitrogen molar ratio; fed-batch culture; human-like collagen II; metabolic-flux analysis (MFA); recombinant Escherichia coli; PATHWAY ANALYSIS; BATCH; GROWTH; HYDROXYAPATITE; CULTIVATION; CULTURE;

D O I：

10.1042/BA20100081

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant Escherichia coli BL2I was used to produce human-like collagen II in fed-batch cultivation. By performing MFA (metabolic-flux analysis), the carbon/nitrogen molar ratios in both the batch and feeding media were optimized for high-level production of human-like collagen II. Three carbon/nitrogen molar ratios in both the batch and feeding media were used in the present study, and the MFA results showed that the optimal carbon/nitrogen molar ratios for the batch and feeding media were 2.36:1 and 5.12:1 respectively, yielding the highest dry-cell density (67.2 g/l dry cell weight) and human-like collagen production (10.8 g/l).

引用

页码：55 / 62

页数：8

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