Live cell calcium imaging at the single ion hit facility of GSI

被引：6

作者：

Du, Guanghua ^{[1
,2
,3
]}

Fischer, Bernd E. ^{[2
]}

Voss, Kay-Obbe ^{[2
]}

机构：

[1] Inst Modern Phys, Lanzhou 730000, Peoples R China

[2] Gesell Schwerionenforsch GSI, D-64291 Darmstadt, Germany

[3] Dept Phys, D-85748 Garching, Germany

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS | 2011年 / 269卷 / 20期

关键词：

Live cell imaging; Microbeam; Calcium imaging; Intracellular free calcium ions; CHARGED-PARTICLE MICROBEAM; MAMMALIAN-CELLS; IRRADIATION; RADIATION; SYSTEM; MICROPROBE;

D O I：

10.1016/j.nimb.2011.02.016

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

To study the fast intracellular calcium response after ion irradiation in living mammalian cells, a live cell calcium imaging set-up was constructed at the targeted cell irradiation facility at GSI. This work introduces the live cell calcium imaging system, shows its performance, an example of the ratio-metric calcium measurement and its application to on-line study calcium response to targeted ion irradiation in human cells. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：2312 / 2316

页数：5

共 19 条

[1] Development of a focused charged particle microbeam for the irradiation of individual cells [J].

Barberet, P ;

Balana, A ;

Incerti, S ;

Michelet-Habchi, C ;

Moretto, P ;

Pouthier, T .

REVIEW OF SCIENTIFIC INSTRUMENTS, 2005, 76 (01) :015101-1

[2] PHOTOBLEACHING OF FURA-2 AND ITS EFFECT ON DETERMINATION OF CALCIUM CONCENTRATIONS [J].

BECKER, PL ;

FAY, FS .

AMERICAN JOURNAL OF PHYSIOLOGY, 1987, 253 (04) :C613-C618

[3] Imaging the hierarchical Ca2+ signalling system in HeLa cells [J].

Bootman, M ;

Niggli, E ;

Berridge, M ;

Lipp, P .

JOURNAL OF PHYSIOLOGY-LONDON, 1997, 499 (02) :307-314

[4]

BREESE MBH, 1992, ANNU REV NUCL PART S, V42, P1

[5] Microirradiation of cells with energetic heavy ions [J].

Dollinger, G ;

Hable, V ;

Hauptner, A ;

Krücken, R ;

Reichart, P ;

Friedl, AA ;

Drexler, G ;

Cremer, T ;

Dietzel, S .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 2005, 231 :195-201

[6]

Du G., 2009, J RADIAT RES, V50, pA94

[7] The absence of an early calcium response to heavy-ion radiation in mammalian cells [J].

Du, Guanghua ;

Fischer, Bernd E. ;

Voss, Kay-O ;

Becker, Gudrun ;

Taucher-Scholz, Gisela ;

Kraft, Gerhard ;

Thiel, Gerhard .

RADIATION RESEARCH, 2008, 170 (03) :316-326

[8]

Folkard M, 1997, INT J RADIAT BIOL, V72, P375, DOI 10.1080/095530097143158

[9] SINGLE-PARTICLE IRRADIATION OF MAMMALIAN-CELLS AT THE RADIOLOGICAL RESEARCH ACCELERATOR FACILITY - INDUCTION OF CHROMOSOMAL CHANGES [J].

GEARD, CR ;

BRENNER, DJ ;

RANDERSPEHRSON, G ;

MARINO, SA .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 1991, 54 (1-3) :411-416

[10] The PTB single ion microbeam for irradiation of living cells [J].

Greif, KD ;

Brede, HJ ;

Frankenberg, D ;

Giesen, U .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 2004, 217 (03) :505-512

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