Neutralization of Staphylococcus aureus Panton Valentine leukocidin by intravenous immunoglobulin in vitro

被引：145
作者：
Gauduchon, V
Cozon, G
Vandenesch, F
Genestier, AL
Eyssade, N
Peyrol, S
Etienne, J
Lina, G
机构：
[1] IFR62 Laennec, INSERM E0230, Ctr Natl Reference Staphylocoques, F-69372 Lyon 08, France
[2] IFR62 Laennec, Ctr Commun Imagerie Laennec, F-69372 Lyon 08, France
来源：
JOURNAL OF INFECTIOUS DISEASES | 2004年 / 189卷 / 02期
关键词：
D O I：
10.1086/380909
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Panton Valentine leukocidin (PVL) may be responsible for pulmonary necrosis in necrotizing Staphylococcus aureus pneumonia, a highly lethal infection. Commercial intravenous immunoglobulin (IVIg) preparations containing antibodies against PVL might have therapeutic value in this setting, as an adjunct to antimicrobial chemotherapy. To test this possibility, we determined anti-PVL antibody titers in commercial IVIg and the capacity of IVIg to prevent the cytopathic effects of PVL in vitro. Specific enzyme-linked immunosorbent assays based on purified recombinant PVL (rPVL) showed that IVIg contained specific anti-PVL antibodies. The cytotoxicity of rPVL and of crude culture supernatants of PVL-producing S. aureus strains were investigated by measuring ethidium-bromide incorporation by polymorphonuclear neutrophils (PMNs) in flow cytometric assays, as well as PMN ultrastructural changes by transmission electron microscopy. IVIg was found to neutralize pore formation and the cytopathic effect of both rPVL and S. aureus culture supernatants.
引用
收藏
页码：346 / 353
页数：8
相关论文
共 21 条
  • [1] STAPHYLOCOCCUS-AUREUS LEUKOCIDIN - A NEW VIRULENCE FACTOR IN CUTANEOUS INFECTIONS - AN EPIDEMIOLOGIC AND EXPERIMENTAL-STUDY
    CRIBIER, B
    PREVOST, G
    COUPPIE, P
    FINCKBARBANCON, V
    GROSSHANS, E
    PIEMONT, Y
    [J]. DERMATOLOGY, 1992, 185 (03) : 175 - 180
  • [2] Community-acquired methicillin-resistant Staphylococcus aureus infections in France:: Emergence of a single clone that produces Panton-Valentine leukocidin
    Dufour, P
    Gillet, Y
    Bes, M
    Lina, G
    Vandenesch, F
    Floret, D
    Etienne, J
    Richet, H
    [J]. CLINICAL INFECTIOUS DISEASES, 2002, 35 (07) : 819 - 824
  • [3] Flow cytometric determination of Panton-Valentine leucocidin S component binding
    Gauduchon, V
    Werner, S
    Prévost, G
    Monteil, H
    Colin, DA
    [J]. INFECTION AND IMMUNITY, 2001, 69 (04) : 2390 - 2395
  • [4] Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients
    Gillet, Y
    Issartel, B
    Vanhems, P
    Fournet, JC
    Lina, G
    Bes, M
    Vandenesch, F
    Piémont, Y
    Brousse, N
    Floret, D
    Etienne, J
    [J]. LANCET, 2002, 359 (9308) : 753 - 759
  • [5] Characterization of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins family
    Gravet, A
    Colin, DA
    Keller, D
    Giradot, R
    Monteil, H
    Prévost, G
    [J]. FEBS LETTERS, 1998, 436 (02) : 202 - 208
  • [6] GROJEC PL, 1981, ZBL BAKT-INT J MED M, V250, P446
  • [7] HORNBECK P, 1997, CURRENT PROTOCOLS MO, V2
  • [8] A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN
    KYTE, J
    DOOLITTLE, RF
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) : 105 - 132
  • [9] Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia
    Lina, G
    Piémont, Y
    Godail-Gamot, F
    Bes, M
    Peter, MO
    Gauduchon, V
    Vandenesch, F
    Etienne, J
    [J]. CLINICAL INFECTIOUS DISEASES, 1999, 29 (05) : 1128 - 1132
  • [10] MUDD S, 1965, BRIT J EXP PATHOL, V46, P455
123