Matrix-assisted laser desorption/ionization mass spectra reflect solution-phase zinc finger peptide complexation

被引：33
作者：
Lehmann, E
Zenobi, R
Vetter, S
机构：
[1] ETH Zurich, Organ Chem Lab, CH-8092 Zurich, Switzerland
[2] ETH Zurich, Biochem Lab, CH-8092 Zurich, Switzerland
来源：
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1999年 / 10卷 / 01期
关键词：
D O I：
10.1016/S1044-0305(98)00116-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The complexation between an 18-residue zinc finger peptide of CCHC type (CCHC = Cys-X-2-Cys-X-4-His-X-4-Cys, X = variable amino acid) from the gag protein p55 of human immunodeficiency virus type 1 (HIV-1) and various transition metal ions was studied by means of circular dichroism spectroscopy and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). A correlation between the complexation behavior in solution and in MALDI-MS could be established. It was shown that MALDI-MS is a fast method suitable for studying metal binding properties of zinc finger complexes. (J Am Soc Mass Spectrom 1999, 10, 27-34) (C) 1999 American Society for Mass Spectrometry.
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页码：27 / 34
页数：8
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