Marked effect of RhoA-specific shRNA-producing plasmids on neurite growth in PC12 cells

被引：10

作者：

Fan, You-Ming ^{[2
,3
,4
]}

Pang, Chi-Pui ^{[1
,2
,3
]}

Harvey, Alan R. ^{[5
]}

Cui, Qi ^{[1
,2
,3
]}

机构：

[1] Chinese Univ Hong Kong, Dept Ophthalmol & Visual Sci, Fac Med, Hong Kong, Hong Kong, Peoples R China

[2] Shantou Univ, Joint Shantou Int Eye Ctr, Shantou, Peoples R China

[3] Shantou Univ, Coll Med, Chinese Univ Hong Kong, Shantou, Peoples R China

[4] Third Mil Med Univ, Dept Pathophysiol & High Altitude Physiol, Chongqing, Peoples R China

[5] Univ Western Australia, Sch Anat & Human Biol, Crawley 6009, Australia

来源：

NEUROSCIENCE LETTERS | 2008年 / 440卷 / 02期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

neurite outgrowth; RhoA; small hairpin RNA; RNA interference; PC12; cells;

D O I：

10.1016/j.neulet.2008.05.045

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In order to promote neurite outgrowth, we constructed a plasmid producing RhoA-specific small hairpin RNA (shRNA) to block RhoA expression and tested its actions in PC12 cells. Our results show that the shRNA-mediated RNA interference (RNAi) successfully suppressed RhoA expression. As a consequence of RhoA knockdown, F-actin levels were significantly reduced and processes were markedly induced. These processes express two neurite markers, neurofilament and Pill tubulin. This study demonstrates that plasmid-producing shRNA specific for RhoA can act as an effective tool to induce neurite outgrowth and further confirms the neurite growth-inhibitory role of RhoA. This shRNA may thus be a useful tool in promoting neurite outgrowth and could be applicable in neural repair after CNS injury. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：170 / 175

页数：6

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