Exosome microarray based on label-free imaging biosensor

被引：2

作者：

Wang, Yifei ^{[1
]}

Yuan, Wang ^{[2
]}

Zhang, Qinming ^{[1
]}

Wang, Yixuan ^{[1
]}

Kimber, Michael ^{[2
]}

Dong, Liang ^{[1
]}

Lu, Meng ^{[1
,3
]}

机构：

[1] Iowa State Univ, Dept Elect & Comp Engn, Ames, IA 50011 USA

[2] Iowa State Univ, Dept Biomed Sci, Ames, IA 50011 USA

[3] Iowa State Univ, Dept Mech Engn, Ames, IA 50011 USA

来源：

2019 IEEE SENSORS | 2019年

基金：

美国国家科学基金会;

关键词：

Exosome Vesicles; microarray; hyperspectral imaging; label-free biosensor; photonic crystal;

D O I：

10.1109/sensors43011.2019.8956490

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Exosome vesicles (EVs) released by macrophages are potential biomarkers for the analysis of immune responses. This study reports a high-throughput EV detection assay developed using a label-free EV microarray. The EV microarray consists of a panel of seven antibodies that are specific to multiple membrane receptors of the target EVs. The EV microarray was fabricated on a photonic crystal (PC) biosensor surface. The hyperspectral imaging approached was implemented to quantify the antibody and EV absorptions on the PC-based microarray. The label-free EV microarray enables low-cost, rapid, and high-throughput characterization of macrophage EVs with a significantly reduced sample volume of 1 mu L.

引用

页数：4

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