Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota

被引：938

作者：

Chang, Chih-Jung ^{[1
,2
,3
,4
,5
,6
]}

Lin, Chuan-Sheng ^{[1
,2
,3
,4
,6
]}

Lu, Chia-Chen ^{[7
]}

Martel, Jan ^{[1
]}

Ko, Yun-Fei ^{[8
,9
]}

Ojcius, David M. ^{[1
,10
]}

Tseng, Shun-Fu ^{[6
]}

Wu, Tsung-Ru ^{[2
,3
,4
]}

Chen, Yi-Yuan Margaret ^{[5
]}

Young, John D. ^{[1
,8
,9
,11
]}

Lai, Hsin-Chih ^{[1
,2
,3
,4
,6
]}

机构：

[1] Chang Gung Univ, Ctr Mol & Clin Immunol, Taoyuan 33302, Taiwan

[2] Chang Gung Univ, Coll Med, Dept Med Biotechnol, Taoyuan 33302, Taiwan

[3] Chang Gung Univ, Coll Med, Lab Sci, Taoyuan 33302, Taiwan

[4] Chang Gung Mem Hosp, Dept Lab Med, Taoyuan 33305, Taiwan

[5] Chang Gung Univ, Dept Microbiol & Immunol, Taoyuan 33302, Taiwan

[6] Chang Gung Univ, Res Ctr Bacterial Pathogenesis, Taoyuan 33302, Taiwan

[7] Fu Jen Catholic Univ, Dept Resp Therapy, New Taipei City 24205, Taiwan

[8] Chang Gung Biotechnol Corp, Taipei 10508, Taiwan

[9] Ming Chi Univ Technol, Biochem Engn Res Ctr, New Taipei City 24301, Taiwan

[10] Univ Pacific, Arthur Dugoni Sch Dent, Dept Biomed Sci, San Francisco, CA 94103 USA

[11] Rockefeller Univ, Lab Cellular Physiol & Immunol, New York, NY 10021 USA

来源：

NATURE COMMUNICATIONS | 2015年 / 6卷

关键词：

DIET-INDUCED OBESITY; HIGH-FAT DIET; INSULIN-RESISTANCE; ADIPOSE-TISSUE; GENE-EXPRESSION; T-CELLS; INFLAMMATION; METABOLISM; ACTIVATION; PREBIOTICS;

D O I：

10.1038/ncomms8489

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Obesity is associated with low-grade chronic inflammation and intestinal dysbiosis. Ganoderma lucidum is a medicinal mushroom used in traditional Chinese medicine with putative anti-diabetic effects. Here, we show that a water extract of Ganoderma lucidum mycelium (WEGL) reduces body weight, inflammation and insulin resistance in mice fed a high-fat diet (HFD). Our data indicate that WEGL not only reverses HFD-induced gut dysbiosis-as indicated by the decreased Firmicutes-to-Bacteroidetes ratios and endotoxin-bearing Proteobacteria levels-but also maintains intestinal barrier integrity and reduces metabolic endotoxemia. The anti-obesity and microbiota-modulating effects are transmissible via horizontal faeces transfer from WEGL-treated mice to HFD-fed mice. We further show that high molecular weight polysaccharides (>300 kDa) isolated from the WEGL extract produce similar anti-obesity and microbiota-modulating effects. Our results indicate that G. lucidum and its high molecular weight polysaccharides may be used as prebiotic agents to prevent gut dysbiosis and obesity-related metabolic disorders in obese individuals.

引用

页数：17

共 70 条

[1] Treg induction by a rationally selected mixture of Clostridia strains from the human microbiota [J].

Atarashi, Koji ;

Tanoue, Takeshi ;

Oshima, Kenshiro ;

Suda, Wataru ;

Nagano, Yuji ;

Nishikawa, Hiroyoshi ;

Fukuda, Shinji ;

Saito, Takuro ;

Narushima, Seiko ;

Hase, Koji ;

Kim, Sangwan ;

Fritz, Joelle V. ;

Wilmes, Paul ;

Ueha, Satoshi ;

Matsushima, Kouji ;

Ohno, Hiroshi ;

Olle, Bernat ;

Sakaguchi, Shimon ;

Taniguchi, Tadatsugu ;

Morita, Hidetoshi ;

Hattori, Masahira ;

Honda, Kenya .

NATURE, 2013, 500 (7461) :232-+

[2] The gut microbiota as an environmental factor that regulates fat storage [J].

Bäckhed, F ;

Ding, H ;

Wang, T ;

Hooper, LV ;

Koh, GY ;

Nagy, A ;

Semenkovich, CF ;

Gordon, JI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (44) :15718-15723

[3] Fecal microbiota transplantation: Indications, methods, evidence, and future directions [J].

Borody T.J. ;

Paramsothy S. ;

Agrawal G. .

Current Gastroenterology Reports, 2013, 15 (8)

[4] Increased intestinal permeability in obese mice:: new evidence in the pathogenesis of nonalcoholic steatohepatitis [J].

Brun, Paola ;

Castagliuolo, Ignazio ;

Di Leo, Vincenza ;

Buda, Andrea ;

Pinzani, Massimo ;

Palu, Giorgio ;

Martines, Diego .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2007, 292 (02) :G518-G525

[5] Gut-derived lipopolysaccharide augments adipose macrophage accumulation but is not essential for impaired glucose or insulin tolerance in mice [J].

Caesar, Robert ;

Reigstad, Christopher S. ;

Backhed, Helene Kling ;

Reinhardt, Christoph ;

Ketonen, Maria ;

Lunden, Gunnel Ostergren ;

Cani, Patrice D. ;

Backhed, Fredrik .

GUT, 2012, 61 (12) :1701-1707

[6] Local and systemic insulin resistance resulting from hepatic activation of IKK-β and NF-κB [J].

Cai, DS ;

Yuan, MS ;

Frantz, DF ;

Melendez, PA ;

Hansen, L ;

Lee, J ;

Shoelson, SE .

NATURE MEDICINE, 2005, 11 (02) :183-190

[7] Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability [J].

Cani, P. D. ;

Possemiers, S. ;

Van de Wiele, T. ;

Guiot, Y. ;

Everard, A. ;

Rottier, O. ;

Geurts, L. ;

Naslain, D. ;

Neyrinck, A. ;

Lambert, D. M. ;

Muccioli, G. G. ;

Delzenne, N. M. .

GUT, 2009, 58 (08) :1091-1103

[8] Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice [J].

Cani, Patrice D. ;

Bibiloni, Rodrigo ;

Knauf, Claude ;

Neyrinck, Audrey M. ;

Delzenne, Nathalle M. ;

Burcelin, Remy .

DIABETES, 2008, 57 (06) :1470-1481

[9] Metabolic endotoxemia initiates obesity and insulin resistance [J].

Cani, Patrice D. ;

Amar, Jacques ;

Iglesias, Miguel Angel ;

Poggi, Marjorie ;

Knauf, Claude ;

Bastelica, Delphine ;

Neyrinck, Audrey M. ;

Fava, Francesca ;

Tuohy, Kieran M. ;

Chabo, Chantal ;

Waget, Aurelie ;

Delmee, Evelyne ;

Cousin, Beatrice ;

Sulpice, Thierry ;

Chamontin, Bernard ;

Ferrieres, Jean ;

Tanti, Jean-Francois ;

Gibson, Glenn R. ;

Casteilla, Louis ;

Delzenne, Nathalie M. ;

Alessi, Marie Christine ;

Burcelin, Remy .

DIABETES, 2007, 56 (07) :1761-1772

[10] QIIME allows analysis of high-throughput community sequencing data [J].

Caporaso, J. Gregory ;

Kuczynski, Justin ;

Stombaugh, Jesse ;

Bittinger, Kyle ;

Bushman, Frederic D. ;

Costello, Elizabeth K. ;

Fierer, Noah ;

Pena, Antonio Gonzalez ;

Goodrich, Julia K. ;

Gordon, Jeffrey I. ;

Huttley, Gavin A. ;

Kelley, Scott T. ;

Knights, Dan ;

Koenig, Jeremy E. ;

Ley, Ruth E. ;

Lozupone, Catherine A. ;

McDonald, Daniel ;

Muegge, Brian D. ;

Pirrung, Meg ;

Reeder, Jens ;

Sevinsky, Joel R. ;

Tumbaugh, Peter J. ;

Walters, William A. ;

Widmann, Jeremy ;

Yatsunenko, Tanya ;

Zaneveld, Jesse ;

Knight, Rob .

NATURE METHODS, 2010, 7 (05) :335-336

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