Development and evaluation of a Mycobacterium bovis interferon-γ enzyme- linked immunospot (ELISpot) assay for detection of bovine tuberculosis

被引：5
作者：
Li, Xin [1 ,2 ,3 ]
Xia, Aihong [1 ]
Xu, Zhengzhong [2 ]
Liu, Jiaying [2 ]
Fu, Shasha [1 ]
Cao, Zhaoli [1 ]
Shen, Yechi [1 ,2 ,3 ]
Xie, Yuqing [1 ,2 ]
Meng, Chuang [2 ]
Chen, Xiang [1 ]
Jiao, Xinan [1 ,2 ,3 ]
机构：
[1] Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control, Jiangsu Key Lab Zoonosis, Important Anim Infect Dis & Zoonoses, Yangzhou 225009, Peoples R China
[2] Yangzhou Univ, Minist Agr & Rural Affairs, Key Lab Prevent & Control Biol Hazard Factors Anim, Yangzhou 225009, Peoples R China
[3] Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou 225009, Peoples R China
来源：
JOURNAL OF DAIRY SCIENCE | 2022年 / 105卷 / 07期
关键词：
bovine tuberculosis; interferon-; ELISpot; monoclonal antibodies; CFP-10-ESAT-6 fusion protein; FUSION PROTEIN; SKIN-TEST; DIAGNOSIS; CATTLE; INFECTION; IDENTIFICATION; BIOMARKERS; ANTIGEN; TESTS;
D O I：
10.3168/jds.2021-21301
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Bovine tuberculosis (bTB) caused by Mycobacterium difficult to control because of the limited ability of the tuberculin skin test (TST) and ancillary IFN-eta release assay to detect all infected animals. In this study, we aimed to develop an efficient assay based on the enzyme-linked immunospot (ELISpot) technique for the diagnosis of bTB, with IFN-eta monoclonal antibodies 3E9 and Bio-labeled 6F8 used as capture and detection an M. bovis-specific antigen, CFP-10-ESAT-6 fusion protein (CE protein), was used. The M. bovis IFN-eta ELISpot assay demonstrated a high level of agreement Fisher Scientific) for detecting bTB. Furthermore, 3 of BOVIGAM ELISA tests, but positive by the ELISpot ELISA+ ELISpot+. These results suggest that the M. infected cattle earlier than the BOVIGAM ELISA test.
引用
收藏
页码：6021 / 6029
页数：9
相关论文
共 46 条
  • [31] Current knowledge and pending challenges in zoonosis caused by Mycobacterium bovis: A review
    Perez-Lago, Laura
    Navarro, Yurena
    Garcia-de-Viedma, Dario
    [J]. RESEARCH IN VETERINARY SCIENCE, 2014, 97 : S94 - S100
  • [32] Role of abattoir monitoring in determining the prevalence of bovine tuberculosis: A systematic review and meta-analysis
    Ramanujam, Harini
    Thiruvengadam, Kannan
    Singaraj, Ranjani
    Palaniyandi, Kannan
    [J]. TRANSBOUNDARY AND EMERGING DISEASES, 2022, 69 (03) : 958 - 973
  • [33] Application of antigenic biomarkers for Mycobacterium tuberculosis
    Rodriguez-Hernandez, Elba
    Quintas-Granados, Laura Itzel
    Flores-Villalva, Susana
    Canto-Alarcon, Jorge Germinal
    Milian-Suazo, Feliciano
    [J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B, 2020, 21 (11): : 856 - 870
  • [34] Interferon-gamma release assay T-SPOT®.TB and HIV-related tuberculosis
    Sattah, Martin V.
    Aye, San San
    Azen, Colleen
    Kort, Jens J.
    Escalante, Patricio
    Jones, Brenda E.
    [J]. INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, 2012, 16 (02) : 281 - 282
  • [35] Bovine Tuberculosis: A Review of Current and Emerging Diagnostic Techniques in View of their Relevance for Disease Control and Eradication
    Schiller, I.
    Oesch, B.
    Vordermeier, H. M.
    Palmer, M. V.
    Harris, B. N.
    Orloski, K. A.
    Buddle, B. M.
    Thacker, T. C.
    Lyashchenko, K. P.
    Waters, W. R.
    [J]. TRANSBOUNDARY AND EMERGING DISEASES, 2010, 57 (04) : 205 - 220
  • [36] Bovine tuberculosis in Europe from the perspective of an officially tuberculosis free country: Trade, surveillance and diagnostics
    Schiller, Irene
    RayWaters, W.
    Vordermeier, H. Martin
    Jemmi, Thomas
    Welsh, Michael
    Keck, Nicolas
    Whelan, Adam
    Gormley, Eamonn
    Boschiroli, Maria Laura
    Moyen, Jean Louis
    Vela, Carmen
    Cagiola, Monica
    Buddle, Bryce M.
    Palmer, Mitchell
    Thacker, Tyler
    Oesch, Bruno
    [J]. VETERINARY MICROBIOLOGY, 2011, 151 (1-2) : 153 - 159
  • [37] Bovine tuberculosis: Effect of the tuberculin skin test on in vitro interferon gamma responses
    Schiller, Irene
    Vordermeier, H. Martin
    Waters, W. Ray
    Whelan, Adam O.
    Coad, Michael
    Gormley, Eamonn
    Buddlee, Bryce M.
    Palmer, Mitchell
    Thacker, Tyler
    McNair, Jim
    Welsh, Michael
    Hewinson, R. Glyn
    Oesch, Bruno
    [J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2010, 136 (1-2) : 1 - 11
  • [38] Sedegah M, 2015, METHODS MOL BIOL, V1325, P197, DOI 10.1007/978-1-4939-2815-6_16
  • [39] Potential of the dual IFN-γ/IL-2 fluorescence-immunospot assay to distinguish different stages in bovine tuberculosis
    Steinbach, Sabine
    Vordermeier, H. Martin
    Jones, Gareth J.
    [J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2019, 217
  • [40] Quantitative genetic analysis of the bTB diagnostic single intradermal comparative cervical test (SICCT)
    Tsairidou, Smaragda
    Brotherstone, Susan
    Coffey, Mike
    Bishop, Stephen C.
    Woolliams, John A.
    [J]. GENETICS SELECTION EVOLUTION, 2016, 48
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