Development and evaluation of a Mycobacterium bovis interferon-γ enzyme- linked immunospot (ELISpot) assay for detection of bovine tuberculosis

被引：5

作者：

Li, Xin ^{[1
,2
,3
]}

Xia, Aihong ^{[1
]}

Xu, Zhengzhong ^{[2
]}

Liu, Jiaying ^{[2
]}

Fu, Shasha ^{[1
]}

Cao, Zhaoli ^{[1
]}

Shen, Yechi ^{[1
,2
,3
]}

Xie, Yuqing ^{[1
,2
]}

Meng, Chuang ^{[2
]}

Chen, Xiang ^{[1
]}

Jiao, Xinan ^{[1
,2
,3
]}

机构：

[1] Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control, Jiangsu Key Lab Zoonosis, Important Anim Infect Dis & Zoonoses, Yangzhou 225009, Peoples R China

[2] Yangzhou Univ, Minist Agr & Rural Affairs, Key Lab Prevent & Control Biol Hazard Factors Anim, Yangzhou 225009, Peoples R China

[3] Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou 225009, Peoples R China

来源：

JOURNAL OF DAIRY SCIENCE | 2022年 / 105卷 / 07期

关键词：

bovine tuberculosis; interferon-; ELISpot; monoclonal antibodies; CFP-10-ESAT-6 fusion protein; FUSION PROTEIN; SKIN-TEST; DIAGNOSIS; CATTLE; INFECTION; IDENTIFICATION; BIOMARKERS; ANTIGEN; TESTS;

D O I：

10.3168/jds.2021-21301

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Bovine tuberculosis (bTB) caused by Mycobacterium difficult to control because of the limited ability of the tuberculin skin test (TST) and ancillary IFN-eta release assay to detect all infected animals. In this study, we aimed to develop an efficient assay based on the enzyme-linked immunospot (ELISpot) technique for the diagnosis of bTB, with IFN-eta monoclonal antibodies 3E9 and Bio-labeled 6F8 used as capture and detection an M. bovis-specific antigen, CFP-10-ESAT-6 fusion protein (CE protein), was used. The M. bovis IFN-eta ELISpot assay demonstrated a high level of agreement Fisher Scientific) for detecting bTB. Furthermore, 3 of BOVIGAM ELISA tests, but positive by the ELISpot ELISA+ ELISpot+. These results suggest that the M. infected cattle earlier than the BOVIGAM ELISA test.

引用

页码：6021 / 6029

页数：9

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