Carbohydrate transporters of the bacterial phosphoenolpyruvate:: sugar phosphotransferase system (PTS)

被引：91

作者：

Siebold, C ^{[1
]}

Flükiger, K ^{[1
]}

Beutler, R ^{[1
]}

Erni, B ^{[1
]}

机构：

[1] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland

来源：

FEBS LETTERS | 2001年 / 504卷 / 03期

关键词：

bacterial phosphotransferase system; cyclic permutation; glucose permease; intein; membrane protein; sugar phosphotransferase system; Escherichia coli;

D O I：

10.1016/S0014-5793(01)02705-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The glucose transporter of Escherichia coli couples translocation with phosphorylation of glucose. The IICBGlc subunit spans the membrane eight times. Split, circularly permuted and cyclized forms of IICBGlc are described. The split variant was 30 times more active when the two proteins were encoded by a dicistronic mRNA than by two genes. The stability and activity of circularly permuted forms was improved when they were expressed as fusion proteins with alkaline phosphatase. Cyclized IICBGlc and IIA(Glc), were produced in vivo by RecA intein-mediated trans-splicing. Purified, cyclized IIA(Glc) and IICBGlc had 100% and 30% of wild-type glucose phosphotransferase activity, respectively. Cyclized IIA(Glc) displayed increased stability against temperature and GuHCl-induced unfolding. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：104 / 111

页数：8

共 67 条

[1] Isolation and characterization of a mutation that alters the substrate specificity of the Escherichia coli glucose permease
Begley, GS
Warner, KA
Arents, JC
Postma, PW
Jacobson, GR
[J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (03) : 940 - 942
[2] Folding and activity of circularly permuted forms of a polytopic membrane protein
Beutler, R
Ruggiero, F
Erni, B
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (04) : 1477 - 1482
[3] Heterodimeric dihydroxyacetone kinase from a ptsI mutant of Escherichia coli
Beutler, R
Kämpfer, U
Schaller, J
Erni, B
[J]. MICROBIOLOGY-SGM, 2001, 147 : 249 - 250
[4] BUHR A, 1993, J BIOL CHEM, V268, P11599
[5] BUHR A, 1992, J BIOL CHEM, V267, P3847
[6] BUHR A, 1994, J BIOL CHEM, V269, P23437
[7] NAD+ AND NADH REGULATE AN ATP-DEPENDENT KINASE THAT PHOSPHORYLATES ENZYME-I OF THE ESCHERICHIA-COLI PHOSPHOTRANSFERASE SYSTEM
DANNELLY, HK
ROSEMAN, S
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (23) : 11274 - 11276
[8] DE GJ, 2001, MOL MICROBIOL, V40, P314
[9] Solution structure of the IIB domain of the glucose transporter of Escherichia coli
Eberstadt, M
Grdadolnik, SG
Gemmecker, G
Kessler, H
Buhr, A
Erni, B
[J]. BIOCHEMISTRY, 1996, 35 (35) : 11286 - 11292
[10] GLUCOSE-SPECIFIC PERMEASE OF THE BACTERIAL PHOSPHOTRANSFERASE SYSTEM - PHOSPHORYLATION AND OLIGOMERIC STRUCTURE OF THE GLUCOSE-SPECIFIC IIGLC-IIIGLC COMPLEX OF SALMONELLA-TYPHIMURIUM
ERNI, B
[J]. BIOCHEMISTRY, 1986, 25 (02) : 305 - 312

← 1 2 3 4 5 6 7 →