Immune responses induced by DNA vaccines encoding Newcastle virus haemagglutinin and/or fusion proteins in maternal antibody-positive commercial broiler chicken

被引：12

作者：

Rajawat, Y. S. ^{[1
]}

Sundaresan, N. R. ^{[2
]}

Ravindra, P. V. ^{[1
]}

Kantaraja, C. ^{[1
]}

Ratta, B. ^{[1
]}

Sudhagar, M. ^{[2
]}

Rai, A. ^{[1
]}

Saxena, V. K. ^{[2
]}

Palia, S. K. ^{[1
]}

Tiwari, A. K. ^{[1
]}

机构：

[1] Indian Vet Res Inst, Mol Biol Lab, Div Anim Biotechnol, Izatnagar 243122, Uttar Pradesh, India

[2] Cent Avian Res Inst, Dis Genet & Biotechnol Lab, Izatnagar, Uttar Pradesh, India

来源：

BRITISH POULTRY SCIENCE | 2008年 / 49卷 / 02期

关键词：

D O I：

10.1080/00071660801939732

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

1.The immune responses induced by recombinant plasmids containing Newcastle disease virus (NDV) F (pVAX.nd.f) or HN (pcDNA.nd.hn) genes separately or in combination in bi-cistronic (pIRES.nd.hn.f) constructs were evaluated in maternal antibody-positive commercial chicks. 2.Immunofluorescence and immunoperoxidase tests demonstrated the expression of both F and HN proteins in Vero cells. Real-time PCR analysis revealed the expression of HN and/or F genes in muscle, peripheral blood mononuclear cells (PBMC), spleen and liver after immunisation. 3.Chicks inoculated intramuscularly thrice (two booster doses) with pVAX.nd.f and pcDNA.nd.hn did not develop detectable haemagglutination inhibiting (HI) antibodies. In contrast, an increase in a NDV-specific cell-mediated immune response was demonstrated. 4.After challenge with virulent NDV, chicks immunised with the recombinant plasmids as well as those in control groups succumbed to Newcastle disease. 5.Based on these results, it is concluded that DNA vaccines containing HN and/or F genes fail to protect commercial chicks, possibly due to interference from maternal antibodies.

引用

页码：111 / 117

页数：7

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