Mixed serum-deprived and normal adipose-derived mesenchymal stem cells against acute lung ischemia-reperfusion injury in rats

被引:0
作者
Sun, Cheuk-Kwan [1 ]
Leu, Steve [2 ,3 ]
Hsu, Shu-Yuan [4 ]
Zhen, Yen-Yi [3 ,5 ]
Chang, Li-Teh [6 ]
Tsai, Ching-Yen [7 ]
Chen, Yung-Lung [5 ]
Chen, Yen-Ta [3 ,8 ]
Tsai, Tzu-Hsien [5 ]
Lee, Fan-Yen [3 ,9 ]
Sheu, Jiunn-Jye [9 ]
Chang, Hsueh-Wen [10 ]
Yip, Hon-Kan [2 ,3 ,5 ,11 ]
机构
[1] I Shou Univ, E Da Hosp, Dept Emergency Med, Kaohsiung, Taiwan
[2] Kaohsiung Chang Gung Mem Hosp, Ctr Translat Res Biomed Sci, Kaohsiung, Taiwan
[3] Chang Gung Univ, Coll Med, Kaohsiung, Taiwan
[4] Chang Gung Univ, Grad Inst Biomed Sci, Coll Med, Dept Anat, Taoyuan, Taiwan
[5] Kaohsiung Chang Gung Mem Hosp, Dept Internal Med, Div Cardiol, Kaohsiung, Taiwan
[6] Meiho Univ, Dept Nursing, Basic Sci, Pingtung, Taiwan
[7] Acad Sinica, Inst Mol Biol, Taipei 115, Taiwan
[8] Kaohsiung Chang Gung Mem Hosp, Div Urol, Kaohsiung, Taiwan
[9] Kaohsiung Chang Gung Mem Hosp, Dept Surg, Div Cardiovasc Surg, Kaohsiung, Taiwan
[10] Natl Sun Yat Sen Univ, Dept Biol Sci, Kaohsiung 80424, Taiwan
[11] Kaohsiung Chang Gung Mem Hosp, Inst Shock Wave Med & Tissue Engn, Kaohsiung, Taiwan
来源
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH | 2015年 / 7卷 / 02期
关键词
Lung ischemia-reperfusion injury; serum-deprived mesenchymal stem cell; cytotherapy; inflammation; oxidative stress; INHALED NITRIC-OXIDE; PROGENITOR CELLS; INTRACORONARY INFUSION; MONONUCLEAR-CELLS; INFARCT SIZE; TRANSPLANTATION; CYCLOSPORINE; INHIBITION; MODULATION; SURFACTANT;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: This study hypothesized that combined serum-deprived (Sd) and healthy (He) adipose-derived mesenchymal stem cell (ADMSC) therapy is superior to either alone in reducing acute lung ischemia-reperfusion injury (ALIRI). Methods: Adult male Sprague-Dawley (SD) rats (n = 30) were equally randomized into group 1 (sham control), group 2 (ALIRI + culture medium), group 3 (ALIRI + intravenous autologous 1.2 x 10(6) He-ADMSCs at 30 minute, 6 h, and 24 h following lung ischemia/reperfusion for 45 minutes/72 hours, respectively), group 4 (ALIRI + 1.2 x 10(6) Sd-ADMSCs at identical time points after ischemia/reperfusion), and group 5 (ALIRI + 1.2 x 10(6) combined Sd-ADMSC/He-ADMSC 1: 1). Results: Blood oxygen saturation (%) was lowest in group 2, lower in groups 3 to 5 than in group 1, and lower in group 5 than in group 1, whereas right ventricular systolic pressure (RVSP) showed a reverse pattern among the five groups (all p < 0.001). Additionally, changes in histological scoring of lung parenchymal damage, inflammatory and apoptotic biomarkers showed identical pattern compared to that of RVSP in all groups (all p < 0.001). Protein expressions of VCAM-1, ICAM-1, oxidative stress, TNF-alpha, nuclear factor-kappa B, and number of CD68 + cells were highest in group 2, higher in groups 3 to 5 than in group 1, and higher in groups 3 and 4 than in group 5, whereas NQO-1 and HO-1 activities and number of CD31 + and vWF + cells showed opposite changes compared with those of inflammatory biomarkers (all p < 0.001). Conclusion: Combined Sd-ADMSC/He-ADMSC therapy offered superior benefit to either option alone in minimizing rodent ALIRI through suppressing oxidative stress and inflammatory reaction.
引用
收藏
页码:209 / 231
页数:23
相关论文
共 33 条
[1]   Clinical Outcome 2 Years After Intracoronary Administration of Bone Marrow-Derived Progenitor Cells in Acute Myocardial Infarction [J].
Assmus, Birgit ;
Rolf, Andreas ;
Erbs, Sandra ;
Elsaesser, Albrecht ;
Haberbosch, Werner ;
Hambrecht, Rainer ;
Tillmanns, Harald ;
Yu, Jiangtao ;
Corti, Roberto ;
Mathey, Detlef G. ;
Hamm, Christian W. ;
Sueselbeck, Tim ;
Tonn, Torsten ;
Dimmeler, Stefanie ;
Dill, Thorsten ;
Zeiher, Andreas M. ;
Schaechinger, Volker .
CIRCULATION-HEART FAILURE, 2010, 3 (01) :89-96
[2]   Reducing ischemia-reperfusion injury in clinical lung transplantation [J].
Bittner, H. B. ;
Binner, C. ;
Dahlberg, P. ;
Mohr, F. W. .
TRANSPLANTATION PROCEEDINGS, 2007, 39 (02) :489-492
[3]   Inhaled nitric oxide for modulation of ischemia-reperfusion injury in lung transplantation [J].
Botha, Phil ;
Jeyakanthan, Mylvaganam ;
Rao, Jagan N. ;
Fisher, Andrew J. ;
Prabhu, Mahesh ;
Dark, John H. ;
Clark, Steven C. .
JOURNAL OF HEART AND LUNG TRANSPLANTATION, 2007, 26 (11) :1199-1205
[4]   Effect of N-acetyl-L-cysteine on lung ischaemia reperfusion injury in a porcine experimental model [J].
Chamogeorgakis, Themistocles P. ;
Kostopanagiotou, Georgia G. ;
Kalimeris, Constantine A. ;
Kabouroglou, George I. ;
Kourtesis, Antonios N. ;
Routsi, Christine I. ;
Dima, Cleanthi C. ;
Toumpoulis, Ioannis K. .
ANZ JOURNAL OF SURGERY, 2008, 78 (1-2) :72-77
[5]   Manipulations of core temperatures in ischemia-reperfusion lung injury in rabbits [J].
Chang, Hung ;
Huang, Kun-Lun ;
Li, Min-Hui ;
Hsu, Ching-Wang ;
Tsai, Shih-Hung ;
Chu, Shi-Jye .
PULMONARY PHARMACOLOGY & THERAPEUTICS, 2008, 21 (02) :285-291
[6]   Mesenchymal progenitor cells differentiate into an endothelial phenotype, enhance vascular density, and improve heart function in a rat cellular cardiomyoplasty model [J].
Davani, S ;
Marandin, A ;
Mersin, N ;
Royer, B ;
Kantelip, B ;
Hervé, P ;
Etievent, JP ;
Kantelip, JP .
CIRCULATION, 2003, 108 (10) :253-258
[7]   Characteristics and outcomes in adult patients receiving mechanical ventilation -: A 28-day international study [J].
Esteban, A ;
Anzueto, A ;
Frutos, F ;
Alía, I ;
Brochard, L ;
Stewart, TE ;
Benito, S ;
Epstein, SK ;
Apezteguía, C ;
Nightingale, P ;
Arroliga, AC ;
Tobin, MJ .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2002, 287 (03) :345-355
[8]   Recruitment Maneuvers for Acute Lung Injury A Systematic Review [J].
Fan, Eddy ;
Wilcox, M. Elizabeth ;
Brower, Roy G. ;
Stewart, Thomas E. ;
Mehta, Sangeeta ;
Lapinsky, Stephen E. ;
Meade, Maureen O. ;
Ferguson, Niall D. .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2008, 178 (11) :1156-1163
[9]   The immune system and cardiac repair [J].
Frangogiannis, Nikolaos G. .
PHARMACOLOGICAL RESEARCH, 2008, 58 (02) :88-111
[10]   Ubiquitin enhances the Th2 cytokine response and attenuates ischemia-reperfusion injury in the lung [J].
Garcia-Covarrubias, Lisardo ;
Manning, Eddie W., III ;
Sorell, Luis T. ;
Pham, Si M. ;
Majetschak, Matthias .
CRITICAL CARE MEDICINE, 2008, 36 (03) :979-982