Expression of Gene uvrA from Acetobacter pasteurianus and Its Tolerance to Acetic Acid in Escherichia coli

被引：3

作者：

Zheng, Yu ^{[1
]}

Chen, Xingjing ^{[1
]}

Wang, Jing ^{[1
]}

Yin, Haisong ^{[1
]}

Wang, Liqing ^{[1
]}

Wang, Min ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Tianjin Key Lab Ind Microbiol, Minist Educ,Coll Biotechnol, Tianjin 300457, Peoples R China

来源：

ADVANCES IN APPLIED BIOTECHNOLOGY, VOL II | 2015年 / 333卷

关键词：

E; coli; uvrA; Acetic acid; Gene expression; DAMAGE RECOGNITION; RESISTANCE; SYSTEM;

D O I：

10.1007/978-3-662-46318-5_18

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The uvrA gene of Acetobacter pasteurianus AC2005 coding for subunit A of the excinuclease ABC complex involved in the nucleotide excision repair mechanism was identified. Gene uvrA was amplified using A. pasteurianus AC2005 genomic DNA as a template. Then the pMV24 plasmid, an expression vector of Acetobacter, was used for constructing the recombinant plasmid pMV24-uvrA. UvrA was expressed in Escherichia coli JM109, and its molecular weight was about 91.1 kDa. With 0.5 % acetic acid shock for 20 and 40 min, the survival rates of recombinant strain E. coli JM109/pMV24-uvrA were 0.48 and 0.056 %, which increased by 17.5 and 10.2 times, respectively, compared with those of E. coli JM109/pMV24. All these demonstrate that the expression of repair excinuclease UvrA could increase the acetic acid tolerance of the strain.

引用

页码：163 / 169

页数：7

共 10 条

[1] Whole-genome analyses reveal genetic instability of Acetobacter pasteurianus [J].

Azuma, Yoshinao ;

Hosoyama, Akira ;

Matsutani, Minenosuke ;

Furuya, Naoko ;

Horikawa, Hiroshi ;

Harada, Takeshi ;

Hirakawa, Hideki ;

Kuhara, Satoru ;

Matsushita, Kazunobu ;

Fujita, Nobuyuki ;

Shirai, Mutsunori .

NUCLEIC ACIDS RESEARCH, 2009, 37 (17) :5768-5783

[2] The uvrA gene is involved in oxidative and acid stress responses in Lactobacillus helveticus CNBL1156 [J].

Cappa, F ;

Cattivelli, D ;

Cocconcelli, PS .

RESEARCH IN MICROBIOLOGY, 2005, 156 (10) :1039-1047

[3] uvrA is an acid-inducible gene involved in the adaptive response to low pH in Streptococcus mutans [J].

Hanna, MN ;

Ferguson, RJ ;

Li, YH ;

Cvitkovitch, DG .

JOURNAL OF BACTERIOLOGY, 2001, 183 (20) :5964-5973

[4] Damage recognition in nucleotide excision DNA repair [J].

Kuper, Jochen ;

Kisker, Caroline .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2012, 22 (01) :88-93

[5] Acetobacter aceti possesses a proton motive force-dependent efflux system for acetic acid [J].

Matsushita, K ;

Inoue, T ;

Adachi, O ;

Toyama, H .

JOURNAL OF BACTERIOLOGY, 2005, 187 (13) :4346-4352

[6] Genome-wide phylogenetic analysis of differences in thermotolerance among closely related Acetobacter pasteurianus strains [J].

Matsutani, Minenosuke ;

Hirakawa, Hideki ;

Saichana, Natsaran ;

Soemphol, Wichai ;

Yakushi, Toshiharu ;

Matsushita, Kazunobu .

MICROBIOLOGY-SGM, 2012, 158 :229-239

[7] A specialized citric acid cycle requiring succinyl-coenzyme a (CoA):: Acetate CoA-transferase (AarC) confers acetic acid resistance on the acidophile Acetobacter acet [J].

Mullins, Elwood A. ;

Francois, Julie A. ;

Kappock, T. Joseph .

JOURNAL OF BACTERIOLOGY, 2008, 190 (14) :4933-4940

[8] Putative ABC transporter responsible for acetic acid resistance in Acetobacter aceti [J].

Nakano, S ;

Fukaya, M ;

Horinouchi, S .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (01) :497-505

[9] Correlation between acetic acid resistance and characteristics of PQQ-dependent ADH in acetic acid bacteria [J].

Trcek, J ;

Toyama, H ;

Czuba, J ;

Misiewicz, A ;

Matsushita, K .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 70 (03) :366-373

[10] 'Close-fitting sleeves': DNA damage recognition by the UvrABC nuclease system [J].

Van Houten, B ;

Croteau, DL ;

DellaVecchia, MJ ;

Wang, H ;

Kisker, C .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2005, 577 (1-2) :92-117

← 1 →