Study of thiazole orange in aptamer-based dye-displacement assays

被引：36

作者：

Pei, Renjun ^{[1
]}

Stojanovic, Milan N. ^{[1
]}

机构：

[1] Columbia Univ, Dept Med, Div Expt Therapeut, New York, NY 10032 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2008年 / 390卷 / 04期

基金：

美国国家科学基金会;

关键词：

aptamers; fluorescence assays; thiazole orange; displacement assays;

D O I：

10.1007/s00216-007-1773-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We screened a series of RNA and DNA aptamers for their ability to serve in the dye displacement assays in which analytes compete with TO dye. We conclude that, while the performance of the TO dye displacement approach is not always predictable, it is still a simple and sensitive assay to detect binding between RNA aptamers and small molecules. In particular, we describe efficient assays for tobramycin and theophylline, with up to 90% displacement of TO observed, and we describe the first aptameric assay for cAMP.

引用

页码：1093 / 1099

页数：7

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