Codon adaptation-based control of protein expression in C. elegans

被引：104

作者：

Redemann, Stefanie ^{[2
]}

Schloissnig, Siegfried ^{[3
]}

Ernst, Susanne ^{[2
]}

Pozniakowsky, Andrey ^{[2
]}

Ayloo, Swathi ^{[2
]}

Hyman, Antony A. ^{[2
]}

Bringmann, Henrik ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Gottingen, Germany

[2] Max Planck Inst Mol Cell Biol & Genet, Dresden, Germany

[3] European Mol Biol Lab, Heidelberg, Germany

来源：

NATURE METHODS | 2011年 / 8卷 / 03期

关键词：

CAENORHABDITIS-ELEGANS; ESCHERICHIA-COLI; SPINDLE POSITION; FORCE GENERATORS; MESSENGER-RNA; SEQUENCE; GENE; USAGE; TRANSLATION; PREDICTION;

D O I：

10.1038/NMETH.1565

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a method to control protein levels under native genetic regulation in Caenorhabditis elegans by using synthetic genes with adapted codons. We found that the force acting on the spindle in C. elegans embryos was related to the amount of the G-protein regulator GPR-1/2. Codon-adapted versions of any C. elegans gene can be designed using our web tool, C. elegans codon adapter.

引用

页码：250 / 252

页数：3

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