Regulation of Stearoyl Coenzyme A Desaturase 1 Gene Promoter in Bovine Mammary Cells

被引:6
作者
di Martino, O. [1 ]
Troiano, A. [1 ]
Addi, L. [2 ]
Guarino, A. [1 ]
Calabro, S. [2 ]
Tudisco, R. [2 ]
Murru, N. [2 ]
Cutrignelli, M. I. [2 ]
Infascelli, F. [2 ]
Calabro, V. [1 ]
机构
[1] Univ Naples Federico II, Dept Biol, Via Cinzia, I-80126 Naples, Italy
[2] Univ Naples Federico II, Dept Vet Med & Anim Prod, I-80126 Naples, Italy
关键词
Bovine mammary cells; Fatty acids; Insulin; Regulation of gene expression; Stearoyl coenzyme a desaturase; FATTY-ACID SECRETION; CONJUGATED LINOLEIC-ACID; SUNFLOWER-SEED OIL; COA DESATURASE; MILK-FAT; NUTRITIONAL REGULATION; LIPID-METABOLISM; LINSEED OIL; DAIRY-COWS; EXPRESSION;
D O I
10.1080/10495398.2015.1022182
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Stearoyl-Coenzyme A desaturase 1 (SCD1) belongs to the fatty acid family of desaturases. In lactating ruminants, the SCD1 protein is highly expressed in the mammary gland and is relevant for the fatty acid composition of milk and dairy products. Bovine mammary epithelial cells (BME-UV1), cultured in vitro, have been proposed as a model to reproduce the biology of the mammary gland. The present study was designed to investigate the responsiveness of bovine SCD1 promoter to serum, insulin, oleic acid, and NFY transcription factor in BME-UV1 cells. A luciferase-based reporter assay was used to monitor the transcriptional activity of the SCD1 promoter region in BME-UV1 cells treated or not with insulin and/or oleic acid. The level of endogenous SCD1 mRNA was evaluated by Real time PCR. Insulin (20 ng/mL) induced a 2.0 to 2.5-fold increase of SCD1 promoter activity. Additionally, the effect of insulin was inhibited by oleic acid, serum components, and NFY enforced expression. Serum and NFY showed no synergistic or additive effect on SCD1 promoter activity suggesting that they repress SCD1 transcription through the same responsive element.
引用
收藏
页码:251 / 259
页数:9
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