Liquid-surface immobilization system and liquid-liquid interface bioreactor: Application to fungal hydrolysis

被引:16
|
作者
Oda, Shinobu [1 ]
Isshiki, Kunio [1 ]
机构
[1] Mercian Corp, Bioresource Labs, Shizuoka 4380078, Japan
关键词
liquid-surface cultivation; interface bioreactor; immobilization; microsphere; lipase; hydrolysis;
D O I
10.1016/j.procbio.2007.09.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Novel fungal cultivation and bioconversion systems are proposed. Spores and mycelia of a fungus suspended in a liquid medium were effectively floated on a liquid surface by the aid of a ballooned microsphere (MS). Many fungi such as Aspergillus and Penicillium formed a thick and physically strong fungus-MS mat on the liquid surface followed by stationary cultivation (LSI). The fungus-MS mat of Absidia coerulea IFO 4423 was overlaid by a solution of 2-ethylhexyl acetate (1) in n-decane (liquid-liquid interface bioreactor, L-L IBR). The strain could efficiently catalyze the hydrolysis of 1 to 2-ethyl-1-hexanol (2). The accumulation of 2 in the L-L IBR was significantly higher than those in emulsion and organic-aqueous two-liquid-phase systems and a formerly reported interface bioreactor (solid-liquid interface bioreactor, S-L IBR). Furthermore, lipase production in the LSI system was also higher than that in a submerged cultivation system. (C) 2007 Elsevier Ltd. All rights reserved.
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页码:1553 / 1560
页数:8
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