Evaluation of epigallocatechin-3-gallate (EGCG) cross-linked collagen membranes and concerns on osteoblasts

被引:69
作者
Chu, Chenyu [1 ]
Deng, Jia [1 ]
Xiang, Lin [1 ,2 ]
Wu, Yingying [1 ,2 ]
Wei, Xiawei [3 ,4 ,5 ]
Qu, Yili [1 ,2 ]
Man, Yi [1 ,2 ]
机构
[1] Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610041, Peoples R China
[2] Sichuan Univ, West China Hosp Stomatol, Dept Oral Implantol, Chengdu 610041, Peoples R China
[3] Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Sichuan, Peoples R China
[4] Sichuan Univ, West China Hosp, Lab Aging Res, Chengdu 610041, Sichuan, Peoples R China
[5] Sichuan Univ, West China Hosp, Collaborat Innovat Ctr Biotherapy, Chengdu 610041, Sichuan, Peoples R China
来源
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2016年 / 67卷
基金
中国国家自然科学基金;
关键词
Epigallocatechin-3-gallate; Collagen membrane; Osteogenesis; Anti-inflammatory; Guide bone regeneration; GREEN-TEA POLYPHENOL; HEPATIC STELLATE CELLS; OSTEOCLAST DIFFERENTIATION; IN-VITRO; (-)-EPIGALLOCATECHIN-3-GALLATE; STABILIZATION; SUPPRESSES; ARTHRITIS; MATRICES; DENATURATION;
D O I
10.1016/j.msec.2016.05.021
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Collagen membranes have ideal biological and mechanical properties for supporting infiltration and proliferation of osteoblasts and play a vital role in guided bone regeneration (GBR). However, pure collagen can lead to inflammation, resulting in progressive bone resorption. Therefore, a method for regulating the level of inflammatory cytokines at surgical sites is paramount for the-healing process. Epigallocatechin-3-gallate (EGCG) is a component extracted from green tea with numerous biological activities including an anti-inflammatory effect. Herein, we present a novel cross-linked collagen membrane containing different concentrations of EGCG (0.0064%, 0.064%, and 0.64%) to regulate the level of inflammatory factors secreted by pre-osteoblast cells; improve cell proliferation; and increase the tensile strength, wettability, and thermal stability of collagen membranes. Scanning election microscope images show that the surfaces of collagen membranes became smoother and the collagen fiber diameters became larger with EGCG treatment. Measurement of the water contact angle demonstrated that introducing EGCG improved membrane wettability. Fourier transform infrared spectroscopy analyses indicated that the backbone of collagen was intact, and the thermal stability was significant improved in differential scanning calorimetry. The mechanical properties of 0.064% and 0.64% EGCG-treated collagen membranes were 1.5-fold greater than those of the control. The extent of cross-linking was significantly increased, as determined by a 2,4,6-trinitrobenzenesulfonic add solution assay. The Cell Counting Kit-8 (CCK-8) and live/dead assays revealed that collagen membrane cross-linked by 0.0064% EGCG induced greater cell proliferation than pure collagen membranes. Additionally, real-time polymerase chain reaction and enzyme-linked immunosorbent assay results showed that EGCG significantly affected the production of inflammatory factors secreted by MC3T3-E1 cells. Taken together, our results indicate that treatment of collagen membranes with appropriate concentrations of EGCG has an anti-inflammatory effect and shows promise for GBR applications. (C) 2016 Published by Elsevier B.V.
引用
收藏
页码:386 / 394
页数:9
相关论文
共 52 条
  • [1] Interaction of collagen with corilagin
    Andrews, ME
    Murali, J
    Muralidharan, C
    Madhulata, W
    Jayakumar, R
    [J]. COLLOID AND POLYMER SCIENCE, 2003, 281 (08) : 766 - 770
  • [2] [Anonymous], 2008, COLLAGEN STRUCTURE M
  • [3] Cross-linking electrospun type II collagen tissue engineering scaffolds with carbodiimide in ethanol
    Barnes, Catherine P.
    Pemble, Charles W.
    Brand, David D.
    Simpson, David G.
    Bowlin, Gary L.
    [J]. TISSUE ENGINEERING, 2007, 13 (07): : 1593 - 1605
  • [4] EGCG remodels mature α-synuclein and amyloid-β fibrils and reduces cellular toxicity
    Bieschke, Jan
    Russ, Jenny
    Friedrich, Ralf P.
    Ehrnhoefer, Dagmar E.
    Wobst, Heike
    Neugebauer, Katja
    Wanker, Erich E.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (17) : 7710 - 7715
  • [5] Adult mesenchymal stem cells for tissue engineering versus regenerative medicine
    Caplan, Arnold I.
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 2007, 213 (02) : 341 - 347
  • [6] Beneficial effects of green tea: A literature review
    Chacko S.M.
    Thambi P.T.
    Kuttan R.
    Nishigaki I.
    [J]. Chinese Medicine, 5 (1)
  • [7] Dahlin C, 1989, Int J Oral Maxillofac Implants, V4, P19
  • [8] INHIBITION OF BONE-RESORPTION IN CULTURE BY (+)-CATECHIN
    DELAISSE, JM
    EECKHOUT, Y
    VAES, G
    [J]. BIOCHEMICAL PHARMACOLOGY, 1986, 35 (18) : 3091 - 3094
  • [9] CONTACT ANGLE HYSTERESIS .4. CONTACT ANGLE MEASUREMENTS ON HETEROGENEOUS SURFACES
    DETTRE, RH
    JOHNSON, RE
    [J]. JOURNAL OF PHYSICAL CHEMISTRY, 1965, 69 (05) : 1507 - &
  • [10] SURFACE HYDROPHOBICITY CHANGES AND HEAT-INDUCED MODIFICATIONS OF ALPHA-LACTALBUMIN
    EYNARD, L
    IAMETTI, S
    RELKIN, P
    BONOMI, F
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1992, 40 (10) : 1731 - 1736