Molecular Detection of Taeniid Eggs in Beetles Collected in an Area Endemic for Taenia solium

被引：11

作者：

Vargas-Calla, Ana ^{[1
]}

Gomez-Puerta, Luis A. ^{[1
]}

Pajuelo, Monica J. ^{[2
]}

Garcia, Hector H. ^{[3
,4
,5
]}

Gonzalez, Armando E. ^{[1
]}

机构：

[1] Univ Nacl Mayor San Marcos, Sch Vet Med, Av Circunvalac Cdra 28, Lima 15021, Peru

[2] Univ Peruana Cayetano Heredia, Lab Infect Dis, Lima, Peru

[3] Univ Peruana Cayetano Heredia, Dept Microbiol, Lima, Peru

[4] Univ Peruana Cayetano Heredia, Ctr Global Hlth, Lima, Peru

[5] Inst Nacl Ciencias Neurol, Cysticercosis Unit, Lima, Peru

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2018年 / 99卷 / 05期

关键词：

COPROPHAGOUS BEETLES; TRANSMISSION; COLEOPTERA; FECES;

D O I：

10.4269/ajtmh.18-0355

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The aim of this study was to demonstrate the presence of Taenia solium eggs in beetles collected from sources within the natural environment through molecular techniques. Fifty-four pools of beetles were collected in three villages in Piura, Peru. DNA was extracted using the FastDNA spin kit for soil. Molecular identification of Taenia species was then performed through partial amplification of the mitochondrial cytochrome C oxidase subunit I gene. Finally, positive samples were sequenced to determine the tapeworm species. Seven positive samples were obtained through polymerase chain reaction amplification. Sequencing confirmed that two samples were from T. solium and three samples were from Taenia hydatigena. The other two samples could not be specifically identified. Our findings demonstrate that dung beetles ingest T. solium and T. hydatigena eggs under natural conditions and suggest that beetles may play a role in the dynamics of transmission of these cestodes.

引用

页码：1198 / 1200

页数：3