Lipid phase control of DNA delivery

被引：61

作者：

Koynova, R ^{[1
]}

Wang, L ^{[1
]}

Tarahovsky, Y ^{[1
]}

MacDonald, RC ^{[1
]}

机构：

[1] Northwestern Univ, Evanston, IL 60208 USA

来源：

BIOCONJUGATE CHEMISTRY | 2005年 / 16卷 / 06期

关键词：

D O I：

10.1021/bc050226x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cationic lipids form nanoscale complexes (lipoplexes) with polyanionic DNA and can be utilized to deliver DNA to cells for transfection. Here we report the correlation between delivery efficiency of these DNA carriers and the mesomorphic phases they form when interacting with anionic membrane lipids. Specifically, formulations that are particularly effective DNA carriers form phases of highest negative interfacial curvature when mixed with anionic lipids, whereas less effective formulations form phases of lower curvature. Structural evolution of the carrier lipid/DNA complexes upon interaction with cellular lipids is hence suggested as a controlling factor in lipid-mediated DNA delivery. A strategy for optimizing lipofection is deduced. The behavior of a highly effective lipoplex formulation, DOTAP/DOPE, is found to conform to this "efficiency formula".

引用

页码：1335 / 1339

页数：5

共 28 条

[1]

AHSLEY GW, 1996, BIOPHYS J, V70, pA88

[2] GEOMETRICAL ASPECTS OF THE FRUSTRATION IN THE CUBIC PHASES OF LYOTROPIC LIQUID-CRYSTALS [J].