Label-free cell-based assays for GPCR screening

被引：76

作者：

Fang, Ye ^{[1
]}

Frutos, Anthony G. ^{[1
]}

Verklereen, Ronald ^{[1
]}

机构：

[1] Corning Inc, Biochem Technol Sci & Technol Div, Corning, NY 14831 USA

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2008年 / 11卷 / 05期

关键词：

G protein-coupled receptor; optical biosensor; resonant waveguide grating biosensor; electrical biosensor; dynamic mass redistribution; impedance; high throughput screening;

D O I：

10.2174/138620708784534789

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

G protein-coupled receptors (GPCRs) have been proven to be the largest family of druggable targets in the human genome. Given the importance of GPCRs as drug targets and the de-orphanization of novel targets, GPCRs are likely to remain the frequent targets of many drug discovery programs. With recent advances in instrumentation and understanding of cellular mechanisms for the signals measured, biosensor-centered label-free cell assay technologies become a very active area for GPCR screening. This article reviews the principles and potential of current label-free cell assay technologies in GPCR drug discovery.

引用

页码：357 / 369

页数：13

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