The nucleocapsid protein of SARS-associated coronavirus inhibits B23 phosphorylation

被引：27

作者：

Zeng, Yingchun ^{[1
]}

Ye, Linbai ^{[1
]}

Zhu, Shengli ^{[1
]}

Zheng, Hong ^{[1
]}

Zhao, Peng ^{[1
]}

Cai, Weijia ^{[1
]}

Su, Liya ^{[1
]}

She, Yinglong ^{[1
]}

Wu, Zhenghui ^{[1
]}

机构：

[1] Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 369卷 / 02期

关键词：

SARS coronavirus; nucleocapsid protein; B23; protein-protein interaction; serine/arginine-rich domain; phosphorylation;

D O I：

10.1016/j.bbrc.2008.01.096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is responsible for SARS infection. Nucleocapsid (N) protein of SARS-CoV encapsidates the viral RNA and plays an important role in virus particle assembly and release. In this study, the N protein of SARS-CoV was found to associate with B23, a phosphoprotein in nucleolus, in vitro-and in vivo. Mapping studies localized the critical P4 sequences for this interaction to amino acid residues 175-210, which included a serine/arginine (SR)-rich domain. In vitro phosphorylation assay showed that the N protein inhibited the B23 phosphorylation at Thr199. (c) 2008 Published by Elsevier Inc.

引用

页码：287 / 291

页数：5

共 50 条

[11] SARS-associated coronavirus infection in teenagers
Yang, GG
Lin, SZ
Liao, KW
Lee, JJ
Wang, LS
EMERGING INFECTIOUS DISEASES, 2004, 10 (02) : 382 - 383
[12] Identification of phosphorylation sites in the nucleocapsid protein (N protein) of SARS-coronavirus
Lin, Liang
Shao, Jianmin
Sun, Maomao
Liu, Jinxiu
Xu, Gongjin
Zhang, Xumin
Xu, Ningzhi
Wang, Rong
Liu, Siqi
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2007, 268 (2-3) : 296 - 303
[13] Peptides from the SARS-associated coronavirus as tags for protein expression and purification
Gruschke, Steffi
Bussmann, Bianca M.
Reiche, Sven
Jassoy, Christian
PROTEIN EXPRESSION AND PURIFICATION, 2008, 61 (02) : 138 - 141
[14] SARS-associated coronavirus quasispecies in individual patients
Xu, DP
Zhang, Z
Wang, FS
NEW ENGLAND JOURNAL OF MEDICINE, 2004, 350 (13): : 1366 - 1367
[15] Effects of a SARS-associated coronavirus vaccine in monkeys
Gao, WT
Tamin, A
Soloff, A
D'Aiuto, L
Nwanegbo, E
Robbins, PD
Bellini, WJ
Barratt-Boyes, S
Gambotto, A
LANCET, 2003, 362 (9399): : 1895 - 1896
[16] Novel rapid immunochromatographic test based on an enzyme immunoassay for detecting nucleocapsid antigen in SARS-associated coronavirus
Kogaki, H
Uchida, Y
Fujii, N
Kurano, Y
Miyake, K
Kido, Y
Kariwa, H
Takashima, I
Tamashiro, H
Ling, AE
Okada, M
JOURNAL OF CLINICAL LABORATORY ANALYSIS, 2005, 19 (04) : 150 - 159
[17] SARS-associated coronavirus replication in cell lines
Kaye, M
Druce, J
Tran, T
Kostecki, R
Chibo, D
Morris, J
Catton, M
Birch, C
EMERGING INFECTIOUS DISEASES, 2006, 12 (01) : 128 - 133
[18] Piezoelectric immunosensor for SARS-associated coronavirus in sputum
Zuo, BL
Li, SM
Guo, Z
Zhang, JF
Chen, CZ
ANALYTICAL CHEMISTRY, 2004, 76 (13) : 3536 - 3540
[19] Proteolysis of SARS-associated coronavirus spike glycoprotein
Simmons, Graham
Rennekamp, Andrew J.
Bates, Paul
NIDOVIRUSES: TOWARD CONTROL OF SARS AND OTHER NIDOVIRUS DISEASES, 2006, 581 : 235 - 240
[20] Profile of specific antibodies to the SARS-associated coronavirus
Li, G
Chen, XJ
Xu, AL
NEW ENGLAND JOURNAL OF MEDICINE, 2003, 349 (05): : 508 - 509

← 1 2 3 4 5 →