The nucleocapsid protein of SARS-associated coronavirus inhibits B23 phosphorylation

被引：27

作者：

Zeng, Yingchun ^{[1
]}

Ye, Linbai ^{[1
]}

Zhu, Shengli ^{[1
]}

Zheng, Hong ^{[1
]}

Zhao, Peng ^{[1
]}

Cai, Weijia ^{[1
]}

Su, Liya ^{[1
]}

She, Yinglong ^{[1
]}

Wu, Zhenghui ^{[1
]}

机构：

[1] Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 369卷 / 02期

关键词：

SARS coronavirus; nucleocapsid protein; B23; protein-protein interaction; serine/arginine-rich domain; phosphorylation;

D O I：

10.1016/j.bbrc.2008.01.096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is responsible for SARS infection. Nucleocapsid (N) protein of SARS-CoV encapsidates the viral RNA and plays an important role in virus particle assembly and release. In this study, the N protein of SARS-CoV was found to associate with B23, a phosphoprotein in nucleolus, in vitro-and in vivo. Mapping studies localized the critical P4 sequences for this interaction to amino acid residues 175-210, which included a serine/arginine (SR)-rich domain. In vitro phosphorylation assay showed that the N protein inhibited the B23 phosphorylation at Thr199. (c) 2008 Published by Elsevier Inc.

引用

页码：287 / 291

页数：5

共 50 条

[1] Cloning, sequencing, expression, and purification of SARS-associated coronavirus nucleocapsid protein for serodiagnosis of SARS
Timani, KA
Ye, L
Ye, LB
Zhu, Y
Wu, ZH
Gong, ZJ
JOURNAL OF CLINICAL VIROLOGY, 2004, 30 (04) : 309 - 312
[2] Peptide domain involved in the interaction between membrane protein and nucleocapsid protein of SARS-associated coronavirus
Fang, XN
Ye, LB
Timani, KA
Li, SS
Zen, YC
Zhao, M
Zheng, H
Wu, ZH
JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2005, 38 (04): : 381 - 385
[3] SARS-associated coronavirus
Holmes, KV
NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20): : 1948 - 1951
[4] Profiles of IgG antibodies to nucleocapsid and spike proteins of the SARS-associated coronavirus in SARS patients
Wang, YB
Chang, ZR
Jing, OY
Wei, HY
Yang, RQ
Chao, YG
Qu, JG
Wang, JW
Hung, T
DNA AND CELL BIOLOGY, 2005, 24 (08) : 521 - 527
[5] Emodin inhibits current through SARS-associated coronavirus 3a protein
Schwarz, Silvia
Wang, Kai
Yu, Wenjing
Sun, Bing
Schwarz, Wolfgang
ANTIVIRAL RESEARCH, 2011, 90 (01) : 64 - 69
[6] Studying on the phosphorylation of the nucleocapsid protein of SARS-coronavirus
Lin, L.
Wang, H.
Li, S.
Zhao, K.
Liu, J.
Liu, S.
MOLECULAR & CELLULAR PROTEOMICS, 2006, 5 (10) : S125 - S125
[7] HIV protease inhibitor nelfinavir inhibits replication of SARS-associated coronavirus
Yamamoto, N
Yang, RG
Yoshinaka, Y
Amari, S
Nakano, T
Cinatl, J
Rabenau, H
Doerr, HW
Hunsmann, G
Otaka, A
Tamamura, H
Fujii, N
Yamamoto, N
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 318 (03) : 719 - 725
[8] Fusogenic mechanism of SARS-associated coronavirus
Liu, S
He, Y
Debnath, AK
Jiang, S
ANTIVIRAL RESEARCH, 2004, 62 (02) : A60 - A60
[9] The genome sequence of the SARS-associated coronavirus
Marra, MA
Jones, SJM
Astell, CR
Holt, RA
Brooks-Wilson, A
Butterfield, YSN
Khattra, J
Asano, JK
Barber, SA
Chan, SY
Cloutier, A
Coughlin, SM
Freeman, D
Girn, N
Griffith, OL
Leach, SR
Mayo, M
McDonald, H
Montgomery, SB
Pandoh, PK
Petrescu, AS
Robertson, AG
Schein, JE
Siddiqui, A
Smailus, DE
Stott, JE
Yang, GS
Plummer, F
Andonov, A
Artsob, H
Bastien, N
Bernard, K
Booth, TF
Bowness, D
Czub, M
Drebot, M
Fernando, L
Flick, R
Garbutt, M
Gray, M
Grolla, A
Jones, S
Feldmann, H
Meyers, A
Kabani, A
Li, Y
Normand, S
Stroher, U
Tipples, GA
Tyler, S
SCIENCE, 2003, 300 (5624) : 1399 - 1404
[10] SEQUENCE OF A PHOSPHORYLATION SITE IN NUCLEOLAR PROTEIN B23
JONES, CE
BUSCH, H
OLSON, MOJ
BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 667 (01) : 209 - 212

← 1 2 3 4 5 →