Liquid Chromatography-Tandem Mass Spectrometry for Determination of Recombinant Thymosin β4 in Rat Plasma

被引：0

作者：

Zhang, Xiaobi ^{[1
]}

Liao, Yingyi ^{[1
]}

Cai, Yonghao ^{[1
]}

机构：

[1] Wenzhou Med Univ, Zhejiang Eye Hosp, Eye Hosp, 270 Xueyuan West Rd, Wenzhou, Zhejiang, Peoples R China

来源：

ANALYTICAL AND QUANTITATIVE CYTOPATHOLOGY AND HISTOPATHOLOGY | 2021年 / 43卷 / 05期

关键词：

high-performance liquid chromatography-tandem mass spectrometry; rat plasma; thymosin beta 4; BETA(4); URINE; MODEL;

D O I：

暂无

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

OBJECTIVE: To develop a fast and sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for determining thymosin beta 4 in rat plasma. STUDY DESIGN: The internal standard analyte was extracted from the rat plasma by cold methanol precipitation. Then 10 mu L of sample supernatant was utilized for detection by UPLC-MS/MS method. The reliability of the method was evaluated by the selectivity, calibration curve, lower limit of quantification, precision and accuracy, matrix effect, recovery, and carry-over. RESULTS: Within the concentration range of 0.1-100 ng/mL, the linear correlation coefficients obtained from the calibration curves were all over 0.997. CONCLUSION: The method has good retention time and peak area repeatability, and the area ratio of residual area to lower limit of quantification is 5.4%, which meets the requirements of the guidelines for nonclinical pharmacokinetic studies of drugs.

引用

页码：320 / 326

页数：7

共 29 条

[1]

[Anonymous], 2010, ANN NY ACAD SCI, V1112, P201

[2] Urine Steroid Metabolomics as a Biomarker Tool for Detecting Malignancy in Adrenal Tumors [J].

Arlt, Wiebke ;

Biehl, Michael ;

Taylor, Angela E. ;

Hahner, Stefanie ;

Libe, Rossella ;

Hughes, Beverly A. ;

Schneider, Petra ;

Smith, David J. ;

Stiekema, Han ;

Krone, Nils ;

Porfiri, Emilio ;

Opocher, Giuseppe ;

Bertherat, Jerome ;

Mantero, Franco ;

Allolio, Bruno ;

Terzolo, Massimo ;

Nightingale, Peter ;

Shackleton, Cedric H. L. ;

Bertagna, Xavier ;

Fassnacht, Martin ;

Stewart, Paul M. .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2011, 96 (12) :3775-3784

[3] Quantification of glucuronidated and sulfated steroids in human urine by ultra-high pressure liquid chromatography quadrupole time-of-flight mass spectrometry [J].

Badoud, Flavia ;

Grata, Elia ;

Boccard, Julien ;

Guillarme, Davy ;

Veuthey, Jean-Luc ;

Rudaz, Serge ;

Saugy, Martial .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2011, 400 (02) :503-516

[4] Thymosin beta 4 regulation of actin in sepsis [J].

Belsky, Justin B. ;

Rivers, Emanuel P. ;

Filbin, Michael R. ;

Lee, Patty J. ;

Morris, Daniel C. .

EXPERT OPINION ON BIOLOGICAL THERAPY, 2018, 18 :193-197

[5] Thymson β4: multiple functions protection, repair and regeneration of the mammalian heart [J].

Bollini, Sveva ;

Riley, Paul R. ;

Smart, Nicola .

EXPERT OPINION ON BIOLOGICAL THERAPY, 2015, 15 :S163-S174

[6] Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma [J].

Chi, Li-Hsing ;

Chang, Wei-Min ;

Chang, Yu-Chan ;

Chan, Yung-Chieh ;

Tai, Chia-Chen ;

Leung, Kam-Wing ;

Chen, Chi-Long ;

Wu, Alexander T. H. ;

Lai, Tsung-Ching ;

Li, Yu-Chuan ;

Hsiao, Michael .

SCIENTIFIC REPORTS, 2017, 7

[7] Thymosin β4 protects C57BL/6 mice from bleomycin-induced damage in the lung [J].

Conte, Enrico ;

Genovese, Tiziana ;

Gili, Elisa ;

Esposito, Emanuela ;

Iemmolo, Maria ;

Fruciano, Mary ;

Fagone, Evelina ;

Pistorio, Maria P. ;

Crimi, Nunzio ;

Cuzzocrea, Salvatore ;

Vancheri, Carlo .

EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 2013, 43 (03) :309-315

[8] Protective effects of thymosin β4 in a mouse model of lung fibrosis [J].

Conte, Enrico ;

Genovese, Tiziana ;

Gili, Elisa ;

Esposito, Emanuela ;

Iemmolo, Maria ;

Fruciano, Mary ;

Fagone, Evelina ;

Pistorio, Maria Provvidenza ;

Crimi, Nunzio ;

Cuzzocrea, Salvatore ;

Vancheri, Carlo .

THYMOSINS IN HEALTH AND DISEASE I, 2012, 1269 :69-73

[9] Thymosin β4-sulfoxide attenuates inflammatory cell infiltration and promotes cardiac wound healing [J].

Evans, Mark A. ;

Smart, Nicola ;

Dube, Karina N. ;

Bollini, Sveva ;

Clark, James E. ;

Evans, Hayley G. ;

Taams, Leonie S. ;

Richardson, Rebecca ;

Levesque, Mathieu ;

Martin, Paul ;

Mills, Kevin ;

Riegler, Johannes ;

Price, Anthony N. ;

Lythgoe, Mark F. ;

Riley, Paul R. .

NATURE COMMUNICATIONS, 2013, 4

[10] Thymosin β4: a multi-functional regenerative peptide. Basic properties and clinical applications [J].

Goldstein, Allan L. ;

Hannappel, Ewald ;

Sosne, Gabriel ;

Kleinman, Hynda K. .

EXPERT OPINION ON BIOLOGICAL THERAPY, 2012, 12 (01) :37-51

← 1 2 3 →