Conjugative transfer, stability and expression of a plasmid encoding a cry1Ac gene in Bacillus cereus group strains

被引：38
作者：
Hu, XM
Hansen, BM [1 ]
Eilenberg, J
Hendriksen, NB
Smidt, L
Yuan, ZM
Jensen, GB
机构：
[1] Natl Environm Res Inst, DK-4000 Roskilde, Denmark
[2] Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China
[3] Royal Vet & Agr Univ, DK-1871 Frederiksberg C, Denmark
[4] Natl Inst Occupat Hlth, DK-2100 Copenhagen, Denmark
来源：
FEMS MICROBIOLOGY LETTERS | 2004年 / 231卷 / 01期
关键词：
conjugation; crylAc; Bacillus thuringiensis; Bacillus cereus; Bacillus mycoides;
D O I：
10.1016/S0378-1097(03)00925-X
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The plasmid pHT73 containing crylAc and tagged with an erythromycin resistance gene was transferred from Bacillus thuringiensis subspecies kurstaki KT0 to several Bacillus cereus group strains by conjugation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and phase contrast microscopy showed that the transconjugants containing plasmid pHT73 could express Cry1Ac toxin and produce bipyramidal crystalline inclusion bodies during sporulation. The study demonstrated that pHT73 could be transferred to B. thuringiensis subsp. kurstaki, several B. cereus strains and Bacillus mycoides. Under non-selective conditions, the stability of the pHT73 plasmid in the transconjugants was found to be 58.2-100% after 100 generations and 4-96% after 200 generations. The variations are mainly caused by the choice of receptor strain. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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页码：45 / 52
页数：8
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