One-pot Production of Lactate-Based Polyesters Using Engineered Microbes Expressing Lactate-Polymerizing Enzyme

被引：2

作者：

Matsumoto, Ken'ichiro ^{[1
]}

Taguchi, Seiichi ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Engn, Div Biotechnol & Macromol Chem, Kita Ku, Sapporo, Hokkaido 0608628, Japan

来源：

KOBUNSHI RONBUNSHU | 2011年 / 68卷 / 05期

关键词：

Poly (lactic acid); PLA; Lactate-Polymerizing Enzyme; Microbial Factory; Chiral Polymer; Biomass; Bio-Based Plastic; PROPIONATE COA-TRANSFERASE; LA-BASED POLYESTERS; ESCHERICHIA-COLI; POLYHYDROXYALKANOATE SYNTHASES; SUBSTRATE-SPECIFICITY; MOLECULAR-WEIGHT; PHA SYNTHASE; IN-VIVO; SATURATION MUTAGENESIS; RALSTONIA-EUTROPHA;

D O I：

10.1295/koron.68.271

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Poly (lactic acid) (PLA) is the most widespread biobased plastic with excellent transparency. Such biobased plastics are expected to be an alternative to petroleum-derived materials. Although PLA is currently produced from fermentative lactate (LA) via chemical polymerization using heavy metal catalysts, we developed a new bioprocess producing LA-based polyester by one-pot reaction. The breakthrough for establishing this process was the discovery of lactate-polymerizing enzyme (LPE). LPE is capable of synthesizing LA-based polyesters with extremely high enantiomer excess. Moreover, the LA-based copolyesters incorporating PHA constituents showed improved flexibility. Therefore, the microbial LA-based polyester is a potent candidate for an environmental friendly plastic.

引用

页码：271 / 280

页数：10

共 41 条

[1] Physical properties and enzymatic degradability of copolymers of (R)-3-hydroxybutyric acid and (S,S)-lactide [J].