"Rules of Engagement" of Protein-Glycoconjugate Interactions: A Molecular View Achievable by using NMR Spectroscopy and Molecular Modeling

被引：59

作者：

Marchetti, Roberta ^{[1
]}

Perez, Serge ^{[2
,3
]}

Arda, Ana ^{[4
]}

Imberty, Anne ^{[5
,6
]}

Jimenez-Barbero, Jesus ^{[4
]}

Silipo, Alba ^{[1
]}

Molinaro, Antonio ^{[1
]}

机构：

[1] Univ Naples Federico II, Dept Chem Sciencest, Via Cintia 4, I-80126 Naples, Italy

[2] CNRS, Dept Mol Pharmacochem, UMR 5063, BP 53, F-38041 Grenoble 9, France

[3] Univ Grenoble Alpes, BP 53, F-38041 Grenoble 9, France

[4] CIC BioGUNE, Bizkaia Technol Pk,Bldg 801A-1, Derio Bizkaia 48160, Spain

[5] CNRS, Ctr Rech, BP 53, F-38041 Grenoble 9, France

[6] Univ Grenoble Macromol Vegetales, UPR 5301, BP 53, F-38041 Grenoble 9, France

来源：

CHEMISTRYOPEN | 2016年 / 5卷 / 04期

关键词：

glycoconjugates; molecular modeling; molecular recognition; NMR spectroscopy; proteins; TRANSFER DIFFERENCE NMR; ANGLE-SPINNING NMR; CARBOHYDRATE-RECOGNITION DOMAIN; UDP-GALACTOPYRANOSE MUTASE; HIGH-AFFINITY LIGANDS; VIRUS-LIKE PARTICLES; HEART CYTOCHROME-C; N-LINKED GLYCANS; STD-NMR; DC-SIGN;

D O I：

10.1002/open.201600024

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Understanding the dynamics of protein-ligand interactions, which lie at the heart of host-pathogen recognition, represents a crucial step to clarify the molecular determinants implicated in binding events, as well as to optimize the design of new molecules with therapeutic aims. Over the last decade, advances in complementary biophysical and spectroscopic methods permitted us to deeply dissect the fine structural details of biologically relevant molecular recognition processes with high resolution. This Review focuses on the development and use of modern nuclear magnetic resonance (NMR) techniques to dissect binding events. These spectroscopic methods, complementing X-ray crystallography and molecular modeling methodologies, will be taken into account as indispensable tools to provide a complete picture of protein-glycoconjugate binding mechanisms related to biomedicine applications against infectious diseases.

引用

页码：274 / 296

页数：23

共 175 条

[1] NMR and modeling studies of protein-carbohydrate interactions:: Synthesis, three-dimensional structure, and recognition properties of a minimum hevein domain with binding affinity for chitooligosaccharides [J].